LOCUS       sequence-119400-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl6 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL6-Myc
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(664..688)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(799..817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(863..914)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(892..912)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtctg tcttcacagt ctggatctga tcttgaagcc
      121 agtctacacc ttcttgcaag ccttctcctt ttatggcatc actagcacaa atatgccagg
      181 gtttatcttt gatgttctct aaacacagca actgagacac ttttacagat gtcactgcat
      241 ctctaagatc cattttattt gcaaagaata agattggaat tcgacggtgt ttaatatctg
      301 gatgattcag aagagtatcg agttcttctt tggccacaac cattcttaat ctatcactac
      361 tatcaatgac aaaaataata gcttggcctt ctttataata gtgttcccag agatttctgt
      421 atcttccttg acctgacatg tcaaacactg taaatgacaa actggatgat ttgaatttct
      481 ctatgctgaa tcctattgtt ggaaggatat tttgagattg agcatttgaa ggtttaagtt
      541 tgttaatgat cgtcgttttg ccactattat ctagcccaag gcacaaaaca tgaacctcct
      601 tcttcttcag gccaagcaag actgaaagtc tgtctagcaa tcccatggtt ctatctcctt
      661 ctaagcctgc ttttttgtac aaacttgttt gattcgagtg tcgacggtat cgataagctt
      721 gatatcgaat tcctgcagcc cgggggatcc gcccgggcta gagcggccgc caccgcggtg
      781 gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcgctagc
      841 ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
      901 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttga
//
