LOCUS       sequence-119401-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4c with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL4C-Myc
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(682..706)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(817..835)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(881..933)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(911..931)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtccc gcttcttctt ctgcttgagg gacttcctgc
      121 gtttcaggat catctcatag agcttgtcca tgccctcggt gaggccctcg ccgatgatgg
      181 cgcacgccgg ctggacgtga taggtggtgg ccgggataag ctcgtgcagc gccagctgct
      241 tctcaatctc tgccaccggc agcgacttgg gcaggtcctg cttgttggcg atgaccagca
      301 gcggcgtgcc ctggttctcg gcgaacttgg tcaccttgtg cagctccgtc ttggcctcct
      361 ccagccggtc cacgtccacc gagtccacca cgtagatgat gccgtccgtg cagcggctgt
      421 aggacttcca cagcggccgc agcttctcct ggccgcccac gtcccagaag tggcagctga
      481 tgcccttggc cgtgccgttg ctcagcttga tcttctcggt gttgaagccg atggtgggca
      541 ccgtgttcac gaactcgttg aacttgagcc ggtagagcac cgtggtcttg ccggccgagt
      601 ccaagcccaa catgacgata tgcagggact ggaaggccga gatgttagag gagatgttgc
      661 ccatggttct atctccttct aagcctgctt ttttgtacaa acttgtttga ttcgagtgtc
      721 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccgc ccgggctaga
      781 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag
      841 cttggcgtaa tcgctagcgg atctgacggt tcactaaacc agctctgctt atatagaacc
      901 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cgga
//
