LOCUS       sequence-119402-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL8A-Myc
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(664..688)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(799..817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(893..913)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcgc ttctccgtga cttcgagtgt tgaataagcc
      121 actgtagggt gatgtcaatg ttgtcctttt ctttgcaaga gatggagtag cagcagatct
      181 ctcggtcctg gatggcagac agattcattt tctcaatcag ctccttctca tccaatgctc
      241 ccggaaggtc tcgcttgtta cccaggacta agaccgggat gccctgcagc tgaggtttgt
      301 ccagtaggtt gtggagctcg ttcttagagg cctcaatctt ctcctggtca gcagcatcca
      361 ccatgtacac gatggcgctc actcctcggc agtagcgctc ccacatgctg cggaaacgcg
      421 gctgtccccc aatgtcccag agcttgatag tcacattccc tttggtgatt ttgcgcatgt
      481 tgaaacccac ggtggggatc atgtcctcgt tgaactgtcc tgacgcgatc acgttgacga
      541 aggtggtctt gcccgagtac tgaagcccga ccagcgtgag ctccatctcc tccttccaga
      601 atagggcctt gaaccagtcc agcagcttgt tgaacaaagc gatcatggtt ctatctcctt
      661 ctaagcctgc ttttttgtac aaacttgttt gattcgagtg tcgacggtat cgataagctt
      721 gatatcgaat tcctgcagcc cgggggatcc gcccgggcta gagcggccgc caccgcggtg
      781 gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcgctagc
      841 ggatctgacg gttcactaaa ccagctctgc tttatataga cctcccaccg tacacgccta
      901 ccgcccattt gcgtcaatgg ggcggagt
//
