LOCUS       sequence-119403-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8b with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL8B-Myc
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(663..687)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(798..816)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(862..913)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(891..911)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcgc ttcttctaga ttttgaatgc tgaataagcc
      121 actgaagtgt gatatctata ttatcctttt ctttgcaaga aattgaatag cagcaaattt
      181 ctctatcctg aatagcagac agattcattt tttcaattag ctgtttctca tccaaggcat
      241 taggaagatc tctcttgttt ccaagcacta gcactggaat tccttgtaac tgtggtttat
      301 ctagaagatt atgtagctca tttcgggaag cttctatctt ttcacgatct gcagcatcta
      361 tcatgtaaac aatagcattg actcctctgc aataccgctc ccacatgctt cgaaatcggg
      421 gttgtcctcc tatgtcccag atctttattg tgacgttacc tttagttacc ttcctcatgt
      481 tgaagcccac tgtgggtatc atatcttcac tgaattgacc tgacgcgatg acattgacga
      541 aggtggtctt gcccgagtac tgcagcccca cgagcgtcag ctccatctct tccttccaga
      601 agagcgaacg gaaccagtcc agcaggcggg agatgagcgc cagcatggtt tatctccttc
      661 taagcctgct tttttgtaca aacttgtttg attcgagtgt cgacggtatc gataagcttg
      721 atatcgaatt cctgcagccc gggggatccg cccgggctag agcggccgcc accgcggtgg
      781 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcgctagcg
      841 gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc
      901 gcccatttgc gtcaatgggg gcggagttgt tacgacattt tggaaagtcc cg
//
