LOCUS       sequence-119406-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4D with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL4D-Myc
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(709..733)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(844..862)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(908..945)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtccc gtctcttctt gccaccccga gctgccttct
      121 tcctcttgag gatcatctca tagaggcgct caaggccctg ctgcaggccc agaccgtcca
      181 cagcgctgca gccttgcaca tgagtgagag tggcggctgc tagctctcgg actgccagcc
      241 tcttctccac ctcagcagcg ctcagtgccc cgggctggtc ctgcttgttg gccagcacca
      301 gcactggcac gccctggttg tccgaggccc ggctgattcg gtgcaactcc accttggctt
      361 cctccagccg ctcagcctcc gcagcgtcca ccacaaacac tagaccgtct gtccggcggg
      421 tataagagcg ccacagtggt cgcagcttct cctgcccccc gacgtcccac acttggaagg
      481 tgatgccacg cgatcccccg aggggcaccc ggatcttctc ggtgttgaag cctttggtgg
      541 ggacactctg gacaaactcc ttgaacttga ggcggtaaag gagggaggtc tttccagcag
      601 agtccagccc aatgaccacg acatgcaggg cttggaagtg gggcaagaag gaggaggcag
      661 tgggcgccat ctcagtcaag tggttcccca tggttctatc tccttctaag cctgcttttt
      721 tgtacaaact tgtttgattc gagtgtcgac ggtatcgata agcttgatat cgaattcctg
      781 cagcccgggg gatccgcccg ggctagagcg gccgccaccg cggtggagct ccagcttttg
      841 ttccctttag tgagggttaa tttcgagctt ggcgtaatcg ctagcggatc tgacggttca
      901 ctaaaccagc tctgcttata tagacctccc acggtacacg cctacggcca tttgcgtcaa
      961 tggggcggaa ttgttacga
//
