LOCUS       sequence-119407-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl11 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARL11-Myc
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(694..718)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(829..847)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(893..944)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(922..942)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcag atctcttgct gtctccgcgc tcagccccat
      121 gggctctcgc ctgcagacac atgcagctgc gagatttcag gaggctccac aggctctgca
      181 gggcctcggg cagcccctcc ccagtgaggg cactgcagcc ccggagctcc cagcggtggt
      241 cctggaatct ctctagactc agcctgtttc tgatcttaag cagcggaagt gcatcaggtg
      301 cctcctgctt gttggccagc accaagaagg ggacgccagc catgttgggg tcgttcagga
      361 cttctgtgag ctcagccgcc gactcgggta agcgggcttc atctgtgctg tccagcacgt
      421 acacgaggat atctgtgcct tccagatagt ccttccagct ggctctgagc ggggcctgcc
      481 ccccaacgtc ccagagagtc agtgacacgt gcccaggagc tttcagaggc tccacgttga
      541 aaccaacagt gggcagggtc tccaccagct ggtggccctt cagcttgtaa aggagcgtgg
      601 tcttgcccgc cgagtccagg cccatcatca ccacctgggc ttccgccttg tgacctctgg
      661 aattcacaga acccatggtt ctatctcctt ctaagcctgc ttttttgtac aaacttgttt
      721 gattcgagtg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
      781 gcccgggcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
      841 gttaatttcg agcttggcgt aatcgctagc ggatctgacg gttcactaaa ccagctctgc
      901 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcg
//
