LOCUS       sequence-119409-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArfrp with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-ARFRP1-Myc
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(709..733)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(844..862)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(908..945)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtccg tgatgtccct ctgccgcggc ggccggtgca
      121 cattccgcac gacacacttc accatccact cgatgccctc gcgcacccct ttgcctgtga
      181 gggccgagca ggcctgggtc aggcaatcgc gcctgccgat cttgctggtg cagtcgctga
      241 aggccgtctt gatgtcaggg attgagaggc acgtctccac atcctgcttg ttggccagca
      301 ccaagacggg gacaccgcac agcgcctcgc tggtcaccac cttctcaaac gcctgcttgg
      361 actcagccag cctctcctcg tcggtggagt caatgacgta gatgacgccg tgacactccg
      421 cataatactt gtcccacaaa gactgcagct cttcctgccc tcctaagtcc cagaacatga
      481 gccgagcctt tcccacatcc acagtgccga tgtttaggcc cacggtggtg gtgattttgg
      541 atagactcat ccccttgtag ttcttgttaa atcgggtttt cgactgctcc aggaaggtcg
      601 tcttcccagc attgtccagg cccaggatca ggatgcagta ctcgtccttc tgaaacatgt
      661 acttgtacaa gcccgacagc agcgtgtaca tggttctatc tccttctaag cctgcttttt
      721 tgtacaaact tgtttgattc gagtgtcgac ggtatcgata agcttgatat cgaattcctg
      781 cagcccgggg gatccgcccg ggctagagcg gccgccaccg cggtggagct ccagcttttg
      841 ttccctttag tgagggttaa tttcgagctt ggcgtaatcg ctagcggatc tgacggttca
      901 ctaaaccagc tctgcttata tagacctccc accgtacacg cctacggcca att
//
