LOCUS       sequence-119411-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSar1 with C-term Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDSHA-SAR1-Myc
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..49)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(700..724)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(835..853)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(899..950)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(928..948)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 attaaggtac cgggccctac agatcctctt cagagatgag tttctgctcc atctcgaatc
       61 gaaccacttt gtacaagaaa gctgggtcgt caatatactg ggagagccag cggaaaccct
      121 cgccgtaacc ttgcctcttg agcacactgc acatgaacac ttccatgggg cgagcattca
      181 gctccttcag ggtcacattc ccctttcctg tggtctgtcc ataaagccca aatatctcac
      241 ggagtttttc ttcactgatt gcatctgttc tgtcaatttt gttacccaag ataaggattg
      301 gcacattgga tattgtttca tcagtcatta aagcattaag ctcaactttg gattccacga
      361 ggcgagaatg atctgcacag tccaccagaa agacaatccc attaattgct gggagataat
      421 ttttccaaac gcgacgtgct tgctcgtgcc caccaagatc aaaagttgta aaggtcattc
      481 cagcaattgt tagctcttct gatgtcggat gtagtgttgg aacatgttgg cccaatctgt
      541 catctttgag catgtgaaga agagtggttt tgcctgcatt atccaaacct aagaatacaa
      601 gttttccaga tttcttgtac agtcctagga actggagcac actgctgaag ccattgtaga
      661 tccactcaaa gatgaaagac atggttctat ctccttctaa gcctgctttt ttgtacaaac
      721 ttgtttgatt cgagtgtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      781 ggatccgccc gggctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
      841 gtgagggtta atttcgagct tggcgtaatc gctagcggat ctgacggttc actaaaccag
      901 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatgggggcg
      961 gagtt
//
