LOCUS       sequence-119414-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl1 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL1-V5-His6
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(684..708)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             734..775
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             785..802
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg
      121 cttagaagga gatagaacca tgggtggctt tttctcaagt atattttcca gtctgtttgg
      181 aactcgggaa atgagaattt taattttggg attagatgga gcaggaaaaa ccacaatttt
      241 gtacagatta caagtgggag aagttgttac tactatacct accattggat ttaatgtaga
      301 gacggtgacg tacaaaaacc ttaaattcca agtctgggat ttaggaggac agacaagtat
      361 caggccatat tggagatgtt actattcaaa cacagatgca gtcatttatg tagtagacag
      421 ttgtgaccga gaccgaattg gcatttccaa atcagagtta gttgccatgt tggaggaaga
      481 agagctgaga aaagccattt tagtggtgtt tgcaaataaa caggacatgg aacaggccat
      541 gacttcctca gagatggcaa attcacttgg gttacctgcc ttgaaggacc gaaaatggca
      601 gatattcaaa acgtcagcaa ccaaaggcac cggccttgat gaggcaatgg aatggttagt
      661 tgaaacatta aaaagcagac aggacccagc tttcttgtac aaagtggttg atctagaggg
      721 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
      781 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
      841 ctagttgcca gccatctgtt gtttgccccc tccccccgt
//
