LOCUS       sequence-119430-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induce human iN conversion from fibroblast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Lau S, Rylander Ottosson D, Jakobsson J, Parmar M
  TITLE     Direct neural conversion from human fibroblasts using 
            self-regulating and nonintegrating viral vectors.
  JOURNAL   Cell Rep. 2014 Dec 11;9(5):1673-80. doi: 
            10.1016/j.celrep.2014.11.017. Epub 2014 Dec 4.
  PUBMED    25482564
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.11.017"; journalName: "Cell Rep"; date: 
            "2014-12-11- 11"; volume: "9"; issue: "5"; pages: "1673-80"
COMMENT     Sequence Label: 1000.pCCLsin.cPPT.hPGK.Brn2.WPRE
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..53)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             202..435
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    507..628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(544..563)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     598..617
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
       61 accttccttc ccgcggcctg ctgccggctc tagagcctct tccgcgtctt cgccttcccg
      121 ggtcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
      181 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
      241 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      421 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
      481 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
      541 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      601 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
      661 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      721 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
//
