LOCUS       sequence-119431-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induce human iN conversion from fibroblast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Lau S, Rylander Ottosson D, Jakobsson J, Parmar M
  TITLE     Direct neural conversion from human fibroblasts using 
            self-regulating and nonintegrating viral vectors.
  JOURNAL   Cell Rep. 2014 Dec 11;9(5):1673-80. doi: 
            10.1016/j.celrep.2014.11.017. Epub 2014 Dec 4.
  PUBMED    25482564
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.11.017"; journalName: "Cell Rep"; date: 
            "2014-12-11- 11"; volume: "9"; issue: "5"; pages: "1673-80"
COMMENT     Sequence Label: 1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4
            Xma_lo...
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..51)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             286..519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    591..712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(628..647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     682..701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
       61 cttccttccc gcggcctgct gccggctcta gataatggca ttcaccgcgt gccttaattc
      121 gaatggcatt caccgcgtgc cttaaacgcg ttggcattca ccgcgtgcct taaatgcatt
      181 ggcattcacc gcgtgcctta acccggtcga gctcggtacc tttaagacca atgacttaca
      241 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
      301 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
      361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
      541 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
      601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      721 ggctctagct atcccg
//
