LOCUS       sequence-119446-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induce human iN conversion from fibroblast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Lau S, Rylander Ottosson D, Jakobsson J, Parmar M
  TITLE     Direct neural conversion from human fibroblasts using 
            self-regulating and nonintegrating viral vectors.
  JOURNAL   Cell Rep. 2014 Dec 11;9(5):1673-80. doi: 
            10.1016/j.celrep.2014.11.017. Epub 2014 Dec 4.
  PUBMED    25482564
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.11.017"; journalName: "Cell Rep"; date: 
            "2014-12-11- 11"; volume: "9"; issue: "5"; pages: "1673-80"
COMMENT     Sequence Label: 1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..60)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             209..442
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    514..635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(551..570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     605..624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
       61 ccagcggacc ttccttcccg cggcctgctg ccggctctag agcctcttcc gcgtcttcgc
      121 cttcccgggt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
      181 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
      241 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      421 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
      481 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
      541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
      661 ccctaactcc gcccatcccg
//
