LOCUS       sequence-119451-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induce human iN conversion from fibroblast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Lau S, Rylander Ottosson D, Jakobsson J, Parmar M
  TITLE     Direct neural conversion from human fibroblasts using 
            self-regulating and nonintegrating viral vectors.
  JOURNAL   Cell Rep. 2014 Dec 11;9(5):1673-80. doi: 
            10.1016/j.celrep.2014.11.017. Epub 2014 Dec 4.
  PUBMED    25482564
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.11.017"; journalName: "Cell Rep"; date: 
            "2014-12-11- 11"; volume: "9"; issue: "5"; pages: "1673-80"
COMMENT     Sequence Label: 1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..54)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             203..436
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    508..629
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(545..564)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     599..618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
       61 gaccttcctt cccgcggcct gctgccggct ctagagcctc ttccgcgtct tcgccttccc
      121 gggtcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
      181 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
      241 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      301 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      361 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      421 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
      481 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
      541 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      601 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
      661 ctccgcccat cccgccccta actccgccca gttccgccca ttct
//
