LOCUS       sequence-119458-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ""Fluorescent reporter for calcium imaging"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Wertz A, Trenholm S, Yonehara K, Hillier D, Raics Z, Leinweber M, 
            Szalay G, Ghanem A, Keller G, Rozsa B, Conzelmann KK, Roska B
  TITLE     PRESYNAPTIC NETWORKS. Single-cell-initiated monosynaptic tracing 
            reveals layer-specific cortical network modules.
  JOURNAL   Science. 2015 Jul 3;349(6243):70-4. doi: 10.1126/science.aab1687.
  PUBMED    26138975
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-07-3- 3"; volume: "349"; issue: 
            "6243"; pages: "70-4"
COMMENT     Sequence Label: pAAV-EF1a-GCaMP6s-WPRE-pGHpA
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..92
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..128
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(132..140,141..155)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             183..239
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     452..473
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(579..600)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(840..859)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 catttcaggt gtcgtgaggt accggatcct ctagaggatc ctgtacaccg cgggctagcg
       61 ccaccatggg ttctcatcat catcatcatc atggtatggc cagcatgact ggtggacagc
      121 aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg gtggactcat
      181 cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac
      241 tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca
      301 tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc
      361 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac
      421 tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      481 ccgggatcac tctcggcatg gacgagctgt ataagggcgg taccggaggg agcatggtga
      541 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      601 taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc
      661 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      721 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      781 acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg
      841 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      901 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      961 agtacaacct gccggaccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct
     1021 ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc
     1081 ggtctctgg
//
