LOCUS       sequence-119463-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Stutz A, Horvath GL, Monks BG, Latz E
  TITLE     ASC speck formation as a readout for inflammasome activation.
  JOURNAL   Methods Mol Biol. 2013;1040:91-101. doi: 
            10.1007/978-1-62703-523-1_8.
  PUBMED    23852599
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2013"; volume: "1040:91-101. doi"; pages: " 
            10.1007/978-1-62703-523-1_8"
COMMENT     Sequence Label: pRP
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             46..645
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(46..65)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     542..562
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgcct ccggtatgac cgagtacaag
       61 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
      121 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
      181 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
      241 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
      301 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
      361 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
      421 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
      481 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
      541 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
      601 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaggatc gatccggcca
      661 ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat
      721 acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac attaccgcca
      781 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      841 agcccatata tggagttccg cgttac
//
