LOCUS       sequence-119500-        4693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase-based reporter for VEGF promoter region (-1000 to -500 
            bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Wood LW, Cox NI, Phelps CA, Lai SC, Poddar A, Talbot C Jr, Mu D
  TITLE     Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of 
            Secretome.
  JOURNAL   Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857.
  PUBMED    26912193
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:19857. doi"; pages: " 
            10.1038/srep19857"
COMMENT     Sequence Label: pGL4.10-VEGFprom(-1000 to -500)
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      545..554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             551..2203
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2247..2368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2258..2277)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2312..2331
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2616..2633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2787..3375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2867..2886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3575..4366,4367..4435))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4540..4588
                     /note="synthetic polyadenylation signal"
     misc_feature    4602..4693
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4642..4661
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccc cagtcccaaa tatgtagctg tttgggaggt cagaaatagg
       61 gggtccagga gcaaactccc cccaccccct ttccaaagcc cattccctct ttagccagag
      121 ccggggtgtg cagacggcag tcactagggg gcgctcggcc accacaggga agctgggtga
      181 atggagcgag cagcgtcttc gagagtgagg acgtgtgtgt ctgtgtgggt gagtgagtgt
      241 gtgcgtgtgg ggttgagggt gttggagcgg ggagaaggcc aggggtcact ccaggattcc
      301 aatagatctg tgtgtccctc tccccacccg tccctgtccg gctctccgcc ttcccctgcc
      361 cccttcaata ttcctagcaa agagggaacg gctctcaggc cctgtccgca cgtaacctca
      421 ctttcctgct ccctcctcgc caatgccccg cgggcgcgtg tctctggaca gagtttccgg
      481 gggcggatgg gtaattttca ggctgtgaac cttggaagct tggcaatccg gtactgttgg
      541 taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
      601 cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc
      661 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
      721 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
      781 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
      841 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
      901 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
      961 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
     1021 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
     1081 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
     1141 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
     1201 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
     1261 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
     1321 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
     1381 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa
     1441 gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     1501 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     1561 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     1621 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     1681 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     1741 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     1801 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     1861 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     1921 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     1981 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     2041 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     2101 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     2161 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg
     2221 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2281 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2341 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2401 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2461 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2521 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2581 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2641 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2701 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3421 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3541 gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac
     3601 cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga
     3661 tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc
     3721 cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa
     3781 gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta
     3841 gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg
     3901 tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc
     3961 gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg
     4021 ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt
     4081 ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt
     4141 cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca
     4201 acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc
     4261 ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac
     4321 ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca
     4381 agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg
     4441 tcctttttca atattattga agcatttatc agggttacta gtacgtctct caaggataag
     4501 taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca
     4561 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     4621 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     4681 agaacatttc tct
//
