LOCUS       sequence-119503-        7390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient human KLF2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: PpyCAG-KLF2-IB
FEATURES             Location/Qualifiers
     source          1..7390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(1270..1843,1844..1846,1847..1855)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1437..1454)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1664..1683
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(2268..2285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2274..2498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2545..2561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2545..2561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2558..2580)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2569..2585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2593..2599,2600..2617,2618..2623))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2638..2659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2718..2913
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2764..2899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2919..3053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3121..3138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3292..3880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3372..3391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4051..4842,4843..4911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4674..4693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    5514..5648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5662..6041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6044..6319
                     /label=chicken beta-actin promoter
     intron          6320..7328
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7252..7274
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa ccatggcgct gagtgaaccc
       61 atcctgccgt ccttctccac tttcgccagc ccgtgccgcg agcgcggcct gcaggagcgc
      121 tggccgcgcg ccgaacccga gtccggcggc accgacgacg acctcaacag cgtgctggac
      181 ttcatcctgt ccatggggct ggatggcctg ggcgccgagg ccgccccgga gccgccgccg
      241 ccgcccccgc cgcctgcgtt ctattacccc gaacccggcg cgcccccgcc ctacagcgcc
      301 cccgcgggtg gcctggtgtc tgagctgctg cgacccgagc tggatgcgcc gccggggccc
      361 gcactgcacg gccgctttct gctggcgccg cccggccgcc tggtcaaggc cgagccccct
      421 gaagcggacg gcggcggcgg ctacggctgc gcccccgggc tgacccgtgg accgcgcggc
      481 ctcaagcgcg agggcgcccc gggcccggcg gcttcgtgca tgcgaggtcc cgggggccgc
      541 cccccgccgc cgcccgacac accgccgctc agccccgacg gccccgcgcg cctgcccgcg
      601 cccggtccgc gcgcctcctt cccgccgcct ttcggtggcc ctggtttcgg cgcgcccggg
      661 cccggcctgc attacgcgcc gcctgcgccc ccagccttcg gtctcttcga cgacgcggcc
      721 gccgccgcgg cagccctggg cctggcgccc cccgccgccc gcggtctcct cacgccgcct
      781 gcgtccccgc tggagctgct ggaggccaag ccaaagcgcg gccgccgctc ttggccccgc
      841 aaacgcaccg ccactcacac ctgcagctac gcgggctgcg gcaagaccta caccaagagt
      901 tcgcatctga aggcgcatct gcgcacgcac acaggtgaga agccctacca ctgcaactgg
      961 gacggctgcg gctggaagtt tgcgcgctca gacgagctca cgcgccacta ccgaaagcac
     1021 acgggccacc ggccattcca gtgccatctg tgcgatcgtg ccttctcgcg ctccgatcac
     1081 ctggcgctgc acatgaaacg gcacatgtag acccatgctt tcttgtacaa agtggtgatt
     1141 cgaggaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     1201 ctggctcaca aataccactg agatcgacgg tatcgagcgg ccgctcgata agcttgatat
     1261 cgaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     1321 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     1381 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     1441 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     1501 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     1561 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     1621 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     1681 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     1741 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     1801 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgcc
     1861 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     1921 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     1981 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     2041 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     2101 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     2161 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     2221 gccctctggt tatgtgtggg agggttaact agataagtaa tgatcagcct cgactgtgcc
     2281 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2341 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2401 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2461 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg
     2521 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2581 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2641 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2701 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2761 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2881 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     2941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     3061 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3481 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3721 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3961 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4021 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4081 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4141 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4201 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4261 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4321 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4381 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4441 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4501 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4561 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4621 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4681 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4741 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4801 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4861 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4921 ttttcaatga ccgctttgga aaaacaaaga ctgtatttcc tggaaattaa tgtttattca
     4981 ataaactgtg tattcagcta tatcacatag tggtgaggct gaaatgaggc gggaagaggc
     5041 ggttggggct taattatatc aatttgggtg gccccacagc gcctccaagg cgccagtcct
     5101 gttttgacaa gttgcctctg gaagcctcta caatgcctct cttctttttc tccagagtaa
     5161 gcggaggcca ggggcccccg gcctctgctt aatactaaaa aaaacagctg ttgtcatagt
     5221 aatgattggg tggaaacatt ccaggcctgg gtggagaggc tttttgcttc ctcttgcaaa
     5281 accacactga cattccaggc ctgggtggag aggctttttg cttcctcttg caaaaccaca
     5341 ctgccctctg gagggcagtt gcctagcaac taactaaaag aggatgtcgc acggccagct
     5401 gcggtcagtt agtcacttcc tgcttaactg acttgacatt ttctatttta agagtcggga
     5461 ggaaaattac tgtgttggag gccctccgcc atcttctgaa gctgaatcga attaacttgt
     5521 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5581 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5641 tctggatctg atatcatcgt cgacattgat tattgactag ttattaatag taatcaatta
     5701 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     5761 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     5821 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggactat ttacggtaaa
     5881 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     5941 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     6001 cttggcagta catctacgta ttagtcatcg ctattaccat gggtcgaggt gagccccacg
     6061 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     6121 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggcgcgcgc caggcggggc
     6181 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     6241 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     6301 gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc
     6361 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     6421 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc tcgtttcttt
     6481 tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc cctttgtgcg ggggggagcg
     6541 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc
     6601 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag
     6661 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct
     6721 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt
     6781 aacccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     6841 ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     6901 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     6961 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     7021 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg gcggagccga aatctgggag
     7081 gcgccgccgc accccctcta gcgggcgcgg gcgaagcggt gcggcgccgg caggaaggaa
     7141 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctcca tctccagcct
     7201 cggggctgcc gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     7261 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     7321 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     7381 tcctcgaatc
//
