LOCUS       sequence-119510-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GPR65-Tango
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg aacagtactt gcatcgaaga gcaacacgac ctcgaccatt
      301 acctgttccc tattgtgtat atttttgtca tcatcgtgtc aattccagca aacataggct
      361 ctctctgcgt cagtttcctt caggccaaaa aggagagcga gcttggcatc tacctgttta
      421 gcctcagtct tagcgacctc ctgtacgcac ttacactccc cctctggata gactatactt
      481 ggaataaaga caactggacc ttcagtccag cactttgtaa aggctcagct ttcctgatgt
      541 acatgaactt ttacagttcc accgcttttc ttacatgcat tgcagtcgac cgctaccttg
      601 ccgtagtcta cccactgaaa ttcttttttc tgagaaccag gagattcgcc ttgatggtga
      661 gcttgagcat ttggattctc gaaaccatat tcaacgccgt tatgctctgg gaagatgaaa
      721 cggtagttga atattgcgat gctgagaagt ccaactttac actgtgctac gataagtatc
      781 ccctggagaa gtggcagatt aacctcaacc tttttcgcac atgcacaggc tacgctatcc
      841 cactggtgac tattctgatt tgtaaccgga aggtctacca ggcggtccgg cacaacaaag
      901 ccactgagaa caaggagaaa aaacggatta taaagctgct ggtgtcaata actgtgacgt
      961 tcgtgttgtg cttc
//
