LOCUS       sequence-119522-       10448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Corynebacter diphtheriae Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10448)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX377 Corynebacter diphtheriae Cas9
FEATURES             Location/Qualifiers
     source          1..10448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1086..1389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1544..1564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1615..1634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1634..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1661..1770
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1772..1796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1825..1851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1855..1875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2945..2959
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     primer_bind     complement(5172..5189)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(5194..5218)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5220..5696
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5419..5438)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     terminator      5697..5744
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5837..6265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5924..5943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6134..6155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6274..6294)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6279..6608
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6459..6594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6521..6540
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6675..7469
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6729..6748)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7339..7358
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7643..7764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7680..7699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7734..7753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    7808..7863
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7862..7881)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      7951..7998
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     8011..8716
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           8099..8471
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(8128..8716)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(8725..8758)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(8926..9513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9005..9024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9526..10317,10318..10386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10149..10168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgaag taccatgtcg gaatcgatgt cggaaccttt
     1921 tctgtggggc tggctgctat tgaagtggat gacgctggaa tgcctattaa gaccctgagt
     1981 ctggtgtcac acattcatga ctcaggactg gatcctgacg agatcaagag cgctgtgacc
     2041 aggctggcaa gctccggaat cgcccggaga acaaggcgcc tgtaccgacg gaagagaagg
     2101 cgcctgcagc agctggataa gttcatccag aggcagggct ggccagtgat cgagctggaa
     2161 gattacagcg accccctgta tccttggaag gtgcgcgccg aactggccgc ttcttatatt
     2221 gctgacgaga aggaacgggg ggagaaactg agtgtggctc tgagacacat cgcaaggcat
     2281 cgcggatgga ggaaccctta cgccaaggtg tctagtctgt atctgccaga tggcccctca
     2341 gacgccttca aggctattag ggaggaaatc aaacgcgcta gcggccagcc tgtgccagag
     2401 actgcaaccg tcgggcagat ggtgaccctg tgcgaactgg gcacactgaa gctgcgagga
     2461 gagggaggag tgctgagtgc acggctgcag cagtcagatt acgcccgcga gatccaggaa
     2521 atttgtcgaa tgcaggagat cggccaggaa ctgtatcgca agatcattga cgtggtgttc
     2581 gcagccgagt ccccaaaggg ctctgcctca agccgggtgg ggaaagatcc tctgcagcca
     2641 ggaaagaaca gagcactgaa agccagcgac gcttttcagc gataccggat tgctgcactg
     2701 atcggcaatc tgagagtcag ggtggatggg gagaagagga ttctgagcgt ggaggagaag
     2761 aacctggtgt tcgaccacct ggtgaatctg actccaaaga aagagcccga atgggtgacc
     2821 atcgccgaaa ttctgggcat cgatcgcggg cagctgatcg gaacagctac tatgaccgac
     2881 gatggagagc gagcaggagc ccgaccccct acacacgata ctaacagaag tattgtgaac
     2941 agccggatcg caccactggt cgactggtgg aaaacagcta gcgcactgga gcagcacgcc
     3001 atggtgaagg cactgtccaa cgccgaagtc gacgattttg attctcccga gggagcaaaa
     3061 gtgcaggcat tctttgccga tctggacgat gacgtccacg ccaagctgga cagcctgcat
     3121 ctgcctgtgg gacgagcagc ttactccgag gacactctgg tcagactgac ccgacggatg
     3181 ctgagtgatg gggtggacct gtataccgcc cggctgcagg agttcggaat tgaacctagc
     3241 tggaccccac ccacaccaag aatcggagag cctgtcggca atccagccgt cgaccgggtg
     3301 ctgaaaacag tgagcagatg gctggaatcc gcaacaaaga cttggggcgc cccagagagg
     3361 gtcatcattg agcacgtgcg cgaaggcttc gtcactgaga aacgcgctcg agaaatggat
     3421 ggggacatga gaaggcgcgc agcccggaac gccaagctgt ttcaggagat gcaggaaaag
     3481 ctgaatgtgc agggcaaacc cagtcgagcc gatctgtgga gataccagtc agtgcagaga
     3541 cagaactgcc agtgtgccta ttgcgggtcc ccaattacct tttctaatag tgaaatggac
     3601 cacatcgtgc ccagagcagg gcagggatcc accaacacaa gggagaatct ggtcgccgtg
     3661 tgccatcgct gtaaccagtc taagggcaat acacccttcg ctatttgggc aaaaaacact
     3721 tctatcgaag gggtcagtgt gaaggaggcc gtggaacgga ccagacattg ggtcactgat
     3781 accggcatga gaagcactga cttcaagaag ttcaccaagg ctgtggtcga gcggtttcag
     3841 agagcaacaa tggatgagga aatcgacgcc agaagcatgg aatccgtcgc ctggatggct
     3901 aatgagctga ggagccgcgt ggctcagcac ttcgcatccc atggaaccac agtcagggtg
     3961 taccgaggca gcctgacagc agaggctcga cgggcatctg ggatcagtgg aaagctgaaa
     4021 ttctttgatg gcgtggggaa gtccaggctg gatagaaggc accatgctat tgacgctgca
     4081 gtgatcgcat tcacctctga ctatgtggcc gaaacactgg ctgtccgctc aaacctgaaa
     4141 cagagccagg cccaccgaca ggaggctcct cagtggagag agttcaccgg caaggatgca
     4201 gagcatcgag cagcttggag agtgtggtgc cagaagatgg aaaaactgag cgccctgctg
     4261 accgaggacc tgcgagatga ccgggtggtc gtgatgtcta acgtgcgact gcggctggga
     4321 aatggcagtg cccacaagga aaccattggc aaactgtcaa aggtgaaact gtcctctcag
     4381 ctgtcagtca gcgatatcga caaagcaagt tcagaggccc tgtggtgtgc tctgaccaga
     4441 gagcccggat tcgatcctaa ggaaggcctg cccgctaacc ctgagagaca catcagggtg
     4501 aatgggacac atgtctacgc cggggacaat attggactgt ttccagtgtc agcaggaagc
     4561 atcgcactga ggggaggata cgcagagctg ggcagctcct tccaccatgc tcgcgtgtat
     4621 aaaattactt ccggcaagaa acccgcattt gccatgctga gggtgtacac catcgatctg
     4681 ctgccttatc gcaaccagga cctgtttagc gtggaactga agccacagac aatgtccatg
     4741 aggcaggctg agaagaaact gcgcgacgct ctggcaactg ggaatgcaga atatctggga
     4801 tggctggtcg tggatgacga gctggtcgtg gatacatcta agattgccac tgaccaggtc
     4861 aaagcagtgg aggccgaact ggggactatc cgccgatggc gggtggatgg attcttttcc
     4921 ccctctaaac tgagactgag gcctctgcag atgtccaagg aggggatcaa gaaagagtcc
     4981 gctcccgaac tgtctaaaat cattgacaga ccaggatggc tgcccgccgt gaacaagctg
     5041 ttctctgatg gaaatgtcac cgtcgtgcgg agagactctc tgggacgcgt gcgactggag
     5101 agtacagccc acctgcctgt cacttggaag gtgcagtaag aattcggtac cgagctcaag
     5161 cttgcggccg cctatagtgt cacctaaatc gtaacccagc tttcttgtac aaagtggtcg
     5221 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     5281 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     5341 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggatgacaa
     5401 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     5461 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     5521 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     5581 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     5641 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctag
     5701 cataacccct tggggcctct aaacgggtct tgaggggttt tttgtctaga agacaatagc
     5761 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5821 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6001 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6061 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6121 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6181 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6241 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6541 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6601 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6661 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6721 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6781 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6841 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6901 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6961 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     7021 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     7081 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     7141 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7201 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7261 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7321 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7381 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7441 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7501 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     7561 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7621 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7741 gtccaaactc atcaatgtat cttatcatgt ctgttcatga agccccttga gcatctgact
     7801 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     7861 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     7921 tagagtttgg caacatatgc ccatatgtaa ctagcataac cccttggggc ctctaaacgg
     7981 gtcttgaggg gttttttgct gaaagcatgc ggaggaaatt ctccttgaag tttccctggt
     8041 gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa
     8101 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
     8161 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
     8221 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
     8281 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
     8341 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
     8401 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
     8461 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
     8521 cgtaaacacg ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg
     8581 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
     8641 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
     8701 tgagcttttt ggaattgcga tcgcataact tcgtatagca tacattatac gaagttataa
     8761 gctcggaacg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     9121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgttacc aatgcttaat
     9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgtc
    10441 cgcgcgtt
//
