LOCUS       sequence-119525-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Dynein-1 LIC1 as a mGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  TITLE     pLVXPuro-mGFP-LIC1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVXPuro-mGFP-LIC1
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             103..354
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     291..312
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
       61 tggagtacaa catacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      121 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      181 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      241 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      301 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagctcgag
      361 gcagctgcag ctatggcggc cgtggggcga gtcggctcct tcggttcttc tccgccggga
      421 ttatcctcga cttacactgg cggccccttg ggcaacgaga tagcgtcggg caacggtggc
      481 gccgcggcag gcgacgacga ggacgggcag aacctttggt cctgcatcct cagcgaggtc
      541 tccacccgct cgcgctccaa gctccctgcg gggaagaacg tgctactgct gggtgaagat
      601 ggagctggaa aaacaagctt aataagaaaa attcagggaa tagaggagta taagaaagga
      661 agaggattgg aatatttgta cttaaatgtg catgatgaa
//
