LOCUS       sequence-119529-       11381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Treponema denticola Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX380 Treponema denticola Cas9
FEATURES             Location/Qualifiers
     source          1..11381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1086..1389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1544..1564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1615..1634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1634..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1661..1770
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1772..1796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1825..1851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1855..1875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6105..6122)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(6127..6151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6153..6629
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6352..6371)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     terminator      6630..6677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6770..7198
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6857..6876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7067..7088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7207..7227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7212..7541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7392..7527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7454..7473
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7608..8402
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7662..7681)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8272..8291
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8576..8697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8613..8632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8667..8686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    8741..8796
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8795..8814)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      8884..8931
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     8944..9649
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           9032..9404
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(9061..9649)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(9658..9691)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(9859..10446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9938..9957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10459..11250,11251..11319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11082..11101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgaaa aaagaaatca aagactactt cctggggctg
     1921 gatgtgggga ctgggagcgt ggggtgggct gtgaccgata ctgactacaa actgctgaag
     1981 gctaaccgaa aagacctgtg gggcatgaga tgcttcgaga cagccgaaac tgctgaggtg
     2041 cggagactgc acaggggagc caggcgccga atcgagcgga gaaagaaacg cattaagctg
     2101 ctgcaggagc tgttctctca ggaaatcgcc aaaaccgatg agggcttctt tcagagaatg
     2161 aaggaaagcc ccttttacgc tgaggacaaa acaatcctgc aggaaaacac tctgttcaat
     2221 gacaaggatt ttgctgataa gacttaccac aaagcatatc ctaccattaa tcatctgatc
     2281 aaggcttgga ttgagaacaa ggtgaaacca gacccccgac tgctgtacct ggcatgtcac
     2341 aacatcatta agaaaagggg acatttcctg tttgaaggcg acttcgattc agagaatcag
     2401 tttgatacca gcatccaggc actgttcgag tatctgcgcg aggacatgga ggtggacatc
     2461 gatgccgaca gccagaaggt caaagagatt ctgaaggata gctccctgaa gaactctgaa
     2521 aaacagagtc ggctgaataa gatcctgggg ctgaagcctt ccgacaaaca gaagaaagcc
     2581 atcacaaacc tgatttctgg aaacaagatc aatttcgccg atctgtacga caatccagat
     2641 ctgaaggacg ctgagaaaaa ctcaatcagc ttctccaagg acgattttga tgcactgagt
     2701 gacgatctgg cctcaattct gggcgacagc tttgaactgc tgctgaaggc caaagctgtc
     2761 tataactgct ctgtgctgag taaggtcatc ggggacgagc agtacctgag cttcgccaag
     2821 gtgaaaatct acgaaaagca caaaaccgat ctgacaaagc tgaaaaacgt gatcaagaaa
     2881 catttcccca aggactacaa gaaggtcttt ggatacaaca agaacgagaa aaacaacaac
     2941 aattactccg gctatgtggg agtctgtaag accaagagta agaaactgat cattaacaac
     3001 tcagtcaacc aggaagattt ctacaagttt ctgaaaacta tcctgtcagc caagagcgag
     3061 atcaaggaag tgaatgacat cctgaccgag attgaaactg gcacctttct gccaaagcag
     3121 atctctaaaa gtaacgcaga gattccctat cagctgagga aaatggagct ggaaaagatc
     3181 ctgtccaatg ccgaaaagca cttctctttt ctgaagcaga aagacgaaaa aggactgtca
     3241 catagcgaga agatcattat gctgctgacc ttcaagatcc cttactatat tggcccaatc
     3301 aacgataatc acaagaaatt ctttcccgac agatgctggg tggtcaagaa agagaaatcc
     3361 ccttctggca agaccacacc atggaacttc tttgatcata tcgacaagga aaaaacagca
     3421 gaggccttca ttacttctag gaccaatttt tgcacttacc tggtgggaga gagcgtcctg
     3481 cctaagtcta gtctgctgta ctccgaatat accgtgctga acgagatcaa caatctgcag
     3541 atcattatcg atggcaagaa tatttgtgac atcaagctga aacagaagat ctacgaggac
     3601 ctgttcaaga agtacaagaa aattacccag aagcagatca gcaccttcat caagcacgaa
     3661 ggcatctgca acaaaaccga tgaagtgatc atcctgggga ttgacaagga atgtacatca
     3721 agcctgaaaa gctacatcga gctgaaaaac attttcggca agcaggtgga tgagatctcc
     3781 actaagaata tgctggagga aattatcaga tgggctacca tctacgacga gggggaagga
     3841 aagaccatcc tgaaaacaaa gatcaaggca gaatacggaa agtattgctc cgacgagcag
     3901 attaagaaaa tcctgaacct gaagttctcc ggctgggggc gactgtctcg gaaatttctg
     3961 gagacagtga ctagtgaaat gcccggcttc tcagaacctg tcaatattat caccgccatg
     4021 agggagacac agaacaatct gatggagctg ctgtcctctg agttcacctt caccgagaac
     4081 attaagaaaa tcaattctgg attcgaagat gccgagaagc agtttagtta cgacggcctg
     4141 gtgaaaccac tgtttctgag tccctcagtc aagaaaatgc tgtggcagac cctgaagctg
     4201 gtgaaagaga ttagccatat cacacaggcc ccccctaaga aaattttcat cgaaatggca
     4261 aagggggccg agctggaacc tgctcggact aagaccagac tgaaaatcct gcaggatctg
     4321 tataacaatt gtaagaacga tgctgacgcc ttcagctcag agatcaaaga cctgagcgga
     4381 aagattgaga acgaagataa tctgaggctg cgctccgaca agctgtacct gtactatact
     4441 cagctgggga aatgcatgta ttgtggaaag ccaattgaga tcggccacgt gttcgatacc
     4501 tcaaactacg atattgacca tatctatccc cagagcaaga tcaaagacga tagcatttcc
     4561 aatcgggtgc tggtctgcag ctcctgtaac aagaacaagg aggacaagta cccactgaaa
     4621 tcagagatcc agagcaagca gcgcggcttc tggaactttc tgcagcgaaa caatttcatt
     4681 tctctggaga agctgaatag actgacaagg gccactccaa tcagtgacga tgagacagcc
     4741 aagtttattg ctaggcagct ggtggaaact cgccaggcta ccaaggtggc cgctaaagtc
     4801 ctggaaaaga tgttccccga gacaaaaatc gtgtacagca aggccgagac tgtctccatg
     4861 ttccggaaca agtttgatat cgtgaagtgc agagaaatta acgattttca ccatgctcac
     4921 gacgcatacc tgaatatcgt ggtcggcaac gtgtataata ccaagttcac aaacaatcct
     4981 tggaacttta tcaaggagaa aagagataat ccaaagattg ctgacaccta caactactat
     5041 aaggtgttcg attatgacgt caaaaggaac aatatcacag catgggagaa ggggaaaact
     5101 attatcaccg tgaaagacat gctgaagaga aacacaccaa tctacactag gcaggcagcc
     5161 tgtaagaaag gggagctgtt caatcagacc attatgaaga aaggactggg ccagcacccc
     5221 ctgaagaaag aaggaccttt ttccaatatc tctaaatacg gcgggtataa caaggtgagc
     5281 gctgcatact atacactgat tgagtatgag gaaaagggca acaaaatccg ctccctggaa
     5341 actattcccc tgtacctggt gaaagatatc cagaaggatc aggacgtcct gaagtcttat
     5401 ctgacagacc tgctggggaa gaaagagttc aagatcctgg tgcccaagat caagatcaac
     5461 agcctgctga agatcaatgg gtttccttgc catattacag gaaaaactaa cgatagtttc
     5521 ctgctgcgcc ctgccgtgca gttttgctgt tcaaacaatg aggtcctgta cttcaagaaa
     5581 attatccggt tttccgaaat ccgctctcag cgagagaaga tcgggaaaac aattagccca
     5641 tacgaggacc tgagcttccg gtcatatatc aaggagaacc tgtggaagaa aactaagaac
     5701 gatgaaatcg gagagaagga attttacgac ctgctgcaga agaaaaacct ggagatctat
     5761 gatatgctgc tgactaagca caaagacacc atctacaaga aacgccctaa ttctgccacc
     5821 attgatatcc tggtgaaggg gaaagagaag ttcaaaagcc tgattatcga aaaccagttt
     5881 gaagtgatcc tggagatcct gaagctgttt tctgcaacac ggaatgtcag tgacctgcag
     5941 catatcggag gcagcaagta ctccggcgtg gccaaaatcg ggaacaagat ctctagtctg
     6001 gataactgta tcctgatcta tcagtccatc accggcatct tcgagaaacg gatcgacctg
     6061 ctgaaggtgt aagaattcgg taccgagctc aagcttgcgg ccgcctatag tgtcacctaa
     6121 atcgtaaccc agctttcttg tacaaagtgg tcgggtggca tccctgtgac ccctccccag
     6181 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     6241 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     6301 tggtatggag caaggggcaa gttgggatga caacctgtag ggcctgcggg gtctattggg
     6361 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     6421 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     6481 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     6541 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     6601 cgtgaaccac tgctcccttc cctgtccttc tagcataacc ccttggggcc tctaaacggg
     6661 tcttgagggg ttttttgtct agaagacaat agcaggcatg ctggggatgc ggtgggctct
     6721 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     6781 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6841 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6901 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6961 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7021 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7081 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7141 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     7201 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7261 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7321 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7381 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7441 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     7501 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     7561 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7621 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7681 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7741 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7801 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7861 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7921 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7981 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     8041 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     8101 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     8161 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     8221 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     8281 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     8341 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     8401 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     8461 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8521 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8581 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8641 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8701 tgtctgttca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     8761 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     8821 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     8881 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagca
     8941 tgcggaggaa attctccttg aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga
     9001 cgcacctctg ttcactggtc cggcgtatta aaacacgata cattgttatt agtacattta
     9061 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
     9121 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
     9181 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
     9241 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
     9301 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
     9361 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
     9421 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata gagcttggac
     9481 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
     9541 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
     9601 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaattg cgatcgcata
     9661 acttcgtata gcatacatta tacgaagtta taagctcgga acgctgcgct cggtcgttcg
     9721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9841 ggccgcgttg ctggcgtttt tccataggct ccgccccctg acgagcatca caaaaatcga
     9901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10021 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
    10081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10261 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10441 tctcaagaag atcctttgtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    10501 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10561 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10621 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10681 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10741 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10801 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10861 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10921 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10981 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11041 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    11101 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11161 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    11221 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    11281 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    11341 ttgaagcatt tatcagggtt attgtctcat gtccgcgcgt t
//
