LOCUS       sequence-119530-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses procollagen 1 alpha 1 as a CFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Stephens DJ, Pepperkok R
  TITLE     Imaging of procollagen transport reveals COPI-dependent cargo 
            sorting during ER-to-Golgi transport in mammalian cells.
  JOURNAL   J Cell Sci. 2002 Mar 15;115(Pt 6):1149-60.
  PUBMED    11884515
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2002-03-15"; volume: "115(Pt 6)"; pages: "1149-60"
COMMENT     Sequence Label: pECFP-N2-COL1A1
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      272..281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacctgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc
      301 cgcggtaccg tcgactgcag gaagcagaca gggccaacgt cgaagccgaa ttcctggtct
      361 ggggcaccaa cgtccaaggg ggccacatcg atgatgggca ggcgggagga cttggtggtt
      421 ttgtattcaa tcactgtctt gccccaggct ccggtgtgac tcgtgcagcc atcgacagtg
      481 acgctgtagg tgaagcggct gttgccctcg gcgcggatct cgatctcgtt ggagccctgg
      541 aggagcaggg ccttcttgag gttgccagtc tgctggtcca tgtaggccac gctgttcttg
      601 cagtggtagg tgatgttctg ggaggcctcg gtggacatca ggcgcaggaa ggtcagctgg
      661 atggccacat cggcagggtc ggagccctgg ccgccatact cgaactggaa tccatcggtc
      721 atgctctcgc cgaaccagac atgcctcttg tcctt
//
