LOCUS       sequence-119547-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NleL (170-782) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Hospenthal MK, Freund SM, Komander D
  TITLE     Assembly, analysis and architecture of atypical ubiquitin chains.
  JOURNAL   Nat Struct Mol Biol. 2013 May;20(5):555-65. doi: 10.1038/nsmb.2547. 
            Epub 2013 Apr 7.
  PUBMED    23563141
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2547";
            journalName: "Nat Struct Mol Biol"; date: "2013-05"; volume: "20"; 
            issue: "5"; pages: "555-65"
COMMENT     Sequence Label: pMCSG17-NleL
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..34
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agatctgggt accgagaacc tgtacttcca atccaatgcg agtcaaggcc gtgcctgcct
       61 gagcaaagct gagcttactg ctgacttaat ttggttgtca gctaaccgaa cgggtgaaga
      121 gtctgctgaa gaattgaatt actctggatg tgacttgtca ggtctaagtc ttgtagggct
      181 gaatttatca tcagtaaatt tttctggagc agtgcttgat gatacagatc tcaggatgag
      241 tgatttgtct caggctgtat tggaaaactg ttcttttaaa aactcgattt tgaatgaatg
      301 taatttttgt tatgctaatt tatctaattg tattattagg gctttgtttg aaaactctaa
      361 tttcagcaat tccaatctta aaaatgcatc atttaaagga tcttcatata tacaatatcc
      421 tccaattttg aacgaggctg atttaacagg agctattata attcctggaa tggttttaag
      481 tggtgctatc ttaggtgatg taaaggagct ctttagtgaa aaaagtaata ccattaatct
      541 aggagggtgt tacatagatc tatctgacat acaggaaaat atattatctg tgttggataa
      601 ctatacaaaa tcaaataaat caattttatt gactatgaat acatctgatg ataagtataa
      661 ccatgataaa gtaagggccg ctgaagaact tatc
//
