LOCUS       sequence-119548-       10616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Roseburia intestinalis Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10616)
  TITLE     Feng Zhang lab bacterial Cas9 orthologs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PX395 Roseburia intestinalis Cas9
FEATURES             Location/Qualifiers
     source          1..10616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     181..1008
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             181..305
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(343..948)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        1086..1389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1390..1593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1544..1564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1615..1634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1634..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1661..1770
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    1772..1796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1825..1851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1855..1875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5340..5357)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(5362..5386)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5388..5864
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5587..5606)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     terminator      5865..5912
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6005..6433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6092..6111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6302..6323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6442..6462)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6447..6776
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6627..6762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6689..6708
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6843..7637
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6897..6916)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7507..7526
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7811..7932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7848..7867)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7902..7921
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    7976..8031
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8030..8049)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      8119..8166
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     8179..8884
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           8267..8639
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(8296..8884)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(8893..8926)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(9094..9681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9173..9192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9694..10485,10486..10554))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10317..10336
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgaga gagaacggga gtgacgaaag gcggaggaat
     1921 atggacgaaa aaatggatta caggattggg ctggacatcg gcatcgcatc agtggggtgg
     1981 gccgtcctgc agaacaattc agacgatgag cctgtgagga ttgtcgacct gggagtgcgc
     2041 attttcgata ccgcagagat cccaaagaca ggcgaaagcc tggcaggacc ccggagagca
     2101 gctcgaacca caaggcgccg actgcggaga aggaaacacc gcctggaccg aatcaagtgg
     2161 ctgttcgaga accaggggct gatcaatatt gacgattttc tgaagagata caacatggcc
     2221 ggactgccag atgtgtacca gctgcggtat gaggctctgg acagaaaact gaccgatgag
     2281 gaactggccc aggtgctgct gcacatcgct aagcatcggg gcttcagaag caccaggaaa
     2341 gccgagacag cagccaagga aaacggggca gtgctgaagg ccacagacga gaatcagaaa
     2401 cgaatgcagg aaaagggata caggacagtg ggcgagatga tctacctgga cgaggccttc
     2461 cggactggct gctcttggag tgagaaaggg tacatcctga ccccccgcaa caaggctgaa
     2521 aattatcagc acacaatgct gcgggcaatg ctggtggagg aagtcaagga gattttcagc
     2581 tcccagcgcc gactgggcaa cgaaaaagcc actgaggaac tggaggaaaa gtacctggag
     2641 atcatgacct cccagcgctc ttttgacctg gggcctggaa tgcagccaga tgggaagccc
     2701 tccccttatg caatggaggg cttctctgac agagtgggga aatgtacttt tctgggggat
     2761 cagggagagc tgaggggcgc taaggggacc tacacagccg aatatttcgt ggctctgcag
     2821 aaaatcaacc acacaaagct ggtcaatcag gacggcgaga caaggaattt cactgaggaa
     2881 gagcggagag ccctgactct gctgctgttt acccagaaag aggtgaagta cgctgcagtc
     2941 cgcaagaaac tgggcctgcc tgaggacatc ctgttctaca acctgaacta caagaaggcc
     3001 gctactaaag aagagcagca gaaggagaac cagaataccg aaaaagccaa gtttatcggg
     3061 atgccatact atcacgatta caagaaatgc ctggaagaga gagtgaagta tctgaccgag
     3121 aacgaagtca gggacctgtt tgatgagatc ggaatgattc tgacttgtta caaaaatgac
     3181 gattcccgca ccgaacgact ggccaagctg ggactggtgc ccatcgagat ggaaggcctg
     3241 ctggcttata ctcctaccaa attccagcat ctgtctatga aggcaatgcg gaacatcatt
     3301 ccctttctgg agaaagggat gacctacgac aaggcttgcg aagaggcagg atatgacttc
     3361 aaagccgata gcaaggggac taaacagaag ctgctgaccg gagagaacgt gaatcagaca
     3421 atcaacgaaa ttactaatcc tgtggtcaaa cgctcagtga gccagacagt gaaggtcatt
     3481 aacgccatca ttcggactta cggcagtcca caggctatca atattgagct ggcaagagaa
     3541 atgtcaaaga cctttgaaga gaggcgcaaa atcaaggggg acatggagaa acggcagaag
     3601 aacaatgaag atgtgaagaa acagattcag gagctgggaa aactgtctcc tacaggccag
     3661 gacatcctga agtacagact gtggcaggag cagcagggga tttgtatgta tagtggaaaa
     3721 accatcccac tggaagagct gttcaagccc ggctacgaca tcgatcacat tctgccctat
     3781 tcaattacat tcgacgatag ctttaggaac aaagtgctgg tcacatccca ggagaacaga
     3841 cagaagggca ataggactcc ttacgagtat atggggaacg acgaacagcg ctggaatgag
     3901 tttgaaacca gggtgaaaac taccatccgc gattacaaga aacagcagaa gctgctgaag
     3961 aaacatttct ctgaagagga aaggagtgag tttaaagaac ggaacctgac agacactaag
     4021 tacatcacaa ccgtgatcta caacatgatc agacagaatc tggagatggc ccccctgaac
     4081 cgccctgaaa agaaaaagca ggtgcgggct gtcaatggcg caattaccgc ctacctgcga
     4141 aaacggtggg ggctgccaca gaagaatcgg gagacagaca cacaccatgc tatggatgca
     4201 gtggtcatcg cctgctgtac cgacggcatg atccagaaaa ttagtagata cacaaaggtg
     4261 agagagaggt gctattcaaa gggaacagag ttcgtcgatg cagagactgg cgaaatcttt
     4321 agacccgagg actacagcag ggccgagtgg gatgaaattt tcggcgtgca catcccaaag
     4381 ccctgggaga catttcgcgc cgaactggac gtccgaatgg gggacgatcc aaagggattc
     4441 ctggacactc atagcgatgt ggctctggag ctggattatc ccgagtacat ctacgaaaac
     4501 ctgcggccta tcttcgtgag cagaatgcca aatcacaagg tcaccggagc agcccatgct
     4561 gacacaattc ggtccccaag acactttaaa gatgagggca tcgtgctgac taagaccgca
     4621 ctgaccgacc tgaaactgga caaggatggg gagatcgacg gatactataa cccccagtcc
     4681 gatctgctgc tgtacgaagc actgaaaaag cagctgctgc tgtatggcaa tgatgccaaa
     4741 aaggccttcg ctcaggactt tcataaaccc aaggccgatg gaactgaggg ccctgtggtc
     4801 aggaaggtga agatccagaa aaagcagacc atgggagtgt tcgtcgactc tggcaacggg
     4861 attgccgaga atggcgggat ggtgcgcatc gatgtgttcc gagtcaacgg caagtactat
     4921 tttgtgcccg tctacaccgc tgacgtggtc aaaaaggtgc tgcctaatag ggccagtaca
     4981 gctcacaagc catacggcga gtggaaagtg atggaggaca aggatttcct gtttagtctg
     5041 tattcacgcg acctgatcca tatcaagtct aaaaaggata tccctattaa gatggtgaac
     5101 ggaggcatgg aggggatcaa ggaaacctac gcatactata ttggagccga catcagcgct
     5161 gcaaatatcc agggcattgc ccacgattcc aggtataaat tccgcggact gggcattcag
     5221 tctctggacg tgctggagaa gtgtcagatc gatgtgctgg gacatgtcag cgtggtccga
     5281 tccgaaaagc ggatgggctt tagctaagaa ttcggtaccg agctcaagct tgcggccgcc
     5341 tatagtgtca cctaaatcgt aacccagctt tcttgtacaa agtggtcggg tggcatccct
     5401 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     5461 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     5521 tggggtggag gggggtggta tggagcaagg ggcaagttgg gatgacaacc tgtagggcct
     5581 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     5641 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     5701 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     5761 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     5821 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctagca taaccccttg
     5881 gggcctctaa acgggtcttg aggggttttt tgtctagaag acaatagcag gcatgctggg
     5941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6001 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6421 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6781 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6841 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6901 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6961 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7021 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7081 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7141 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7201 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7261 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7321 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7381 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7441 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7501 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7561 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7621 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     7681 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7741 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7801 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7921 caatgtatct tatcatgtct gttcatgaag ccccttgagc atctgacttc tggctaataa
     7981 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     8041 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     8101 acatatgccc atatgtaact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     8161 tttttgctga aagcatgcgg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
     8221 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
     8281 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
     8341 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
     8401 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
     8461 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
     8521 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
     8581 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
     8641 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
     8701 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
     8761 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
     8821 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
     8881 aattgcgatc gcataacttc gtatagcata cattatacga agttataagc tcggaacgct
     8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc ccctgacgag
     9121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     9301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9661 cgcagaaaaa aaggatctca agaagatcct ttgttaccaa tgcttaatca gtgaggcacc
     9721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgtccg cgcgtt
//
