LOCUS       sequence-119564-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GRPR-Tango
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgttta aacttaagct tggtaccgag ctcggatcca
      121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc gccaccatga
      181 agacgatcat cgccctgagc tacatcttct gcctggtatt cgccgactac aaggacgatg
      241 atgacgccag catcgatatg gccctcaacg attgctttct gttgaatctg gaagtggacc
      301 actttatgca ctgcaatatt tcatcccatt ctgctgatct gcctgtgaat gacgactggt
      361 ctcatccggg aattctgtac gtgattcctg cggtctacgg ggtaataatt ctcattgggc
      421 tgattggcaa cattacgctg atcaagatct tctgcaccgt caaatcaatg aggaacgtcc
      481 ctaacctgtt tataagttct ctggcattgg gtgacctcct tctgctgata acatgtgccc
      541 ctgttgatgc ctctagatac ctggcggaca gatggctgtt tgggagaata ggttgtaaac
      601 tgattccctt cattcagctg acctcagtgg gagtttctgt cttcaccctc acagcacttt
      661 ctgcggatcg ctacaaagct atcgtgaggc ccatggatat ccaggcatcc catgccctca
      721 tgaagatctg cttgaaggcg gcgttcattt ggattatcag catgcttttg gccatccccg
      781 aagcagtatt ttccgacctc cacccatttc acgaagaaag cacgaaccag acttttattt
      841 cctgcgcccc atacccccac tccaatgagc tgcaccctaa gattcattct atggcctcat
      901 tcctcgtctt ttacgtgatc cccttgagta taatctccgt ttactactac ttcatagcga
      961 agaacctgat tcagtca
//
