LOCUS       sequence-119568-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hSara with C-term V5 and His6 tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pET-DEST42-SARA-V5-His6
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(677..701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             721..762
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             772..789
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(846..864)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      860..907
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cctctagaat aattttgttt aactttaaga aggaatatca caagtttgta caaaaaagca
       61 ggcttagaag gagatagaac catgtccttc atatttgatt ggatttacag tggtttcagc
      121 agtgtgctac agtttttagg attatataag aaaactggta aactggtatt tcttggattg
      181 gataatgcag gaaaaacaac attgctacac atgctaaaag atgacagact tggacaacat
      241 gtcccaacat tacatcccac ttccgaagaa ctgaccattg ctggcatgac gtttacaact
      301 tttgatctgg gtggacatgt tcaagctcga agagtgtgga aaaactacct tcctgctatc
      361 aatggcattg tatttctggt ggattgtgca gaccacgaaa ggctgttaga gtcaaaagaa
      421 gaacttgatt cactaatgac agatgaaacc attgctaatg tgcctatact gattcttggg
      481 aataagatcg acagacctga agccatcagt gaagagaggt tgcgagagat gtttggttta
      541 tatggtcaga caacaggaaa ggggagtata tctctgaaag aactgaatgc ccgaccctta
      601 gaagttttca tgtgtagtgt gctcaaaaga caaggttacg gagaaggctt ccgctggatg
      661 gcacagtaca ttgatgaccc agctttcttg tacaaagtgg tgatcaattc gaagcttgaa
      721 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac
      781 catcaccatt gagtttgatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      841 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaaccgg gtcttgaggg
      901 gtttttttgc tgaaagggag gaactatatc c
//
