LOCUS       sequence-119569-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf3 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARF3-V5-His6
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(574..598)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             624..665
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             675..692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(715..732)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    721..832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgggcaatat ctttggaaac cttctcaaga
       61 gcctgattgg gaagaaggag atgcgcatcc tgatggtggg cctggatgcc gcaggaaaga
      121 ccaccatcct atacaagctg aaactggggg agatcgtcac caccatccct accattgggt
      181 tcaatgtgga gacagtggag tataagaaca tcagctttac agtgtgggat gtgggtggcc
      241 aggacaagat tcgacccctc tggagacact acttccagaa cacccaaggg ttgatatttg
      301 tggtcgacag caatgatcgg gagcgagtaa atgaggcccg ggaagagctg atgagaatgc
      361 tggcggagga cgagctccgg gatgctgtac tccttgtctt tgcaaacaaa caggatctgc
      421 ctaatgctat gaacgctgct gagatcacag acaagctggg cctgcattcc cttcgtcacc
      481 gtaactggta cattcaggcc acctgtgcca ccagcgggga cgggctgtac gaaggcctgg
      541 actggctggc caatcagctc aaaaacaaga aggacccagc tttcttgtac aaagtggttg
      601 atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
      661 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
      721 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      781 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      841 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggga
//
