LOCUS       sequence-119573-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl8b with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL8B-V5-His6
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(588..612)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             638..679
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(729..746)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    735..960
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gataaaccat gctggcgctc atctcccgcc tgctggactg
       61 gttccgttcg ctcttctgga aggaagagat ggagctgacg ctcgtggggc tgcagtactc
      121 gggcaagacc accttcgtca atgtcatcgc gtcaggtcaa ttcagtgaag atatgatacc
      181 cacagtgggc ttcaacatga ggaaggtaac taaaggtaac gtcacaataa agatctggga
      241 cataggagga caaccccgat ttcgaagcat gtgggagcgg tattgcagag gagtcaatgc
      301 tattgtttac atgatagatg ctgcagatcg tgaaaagata gaagcttccc gaaatgagct
      361 acataatctt ctagataaac cacagttaca aggaattcca gtgctagtgc ttggaaacaa
      421 gagagatctt cctaatgcct tggatgagaa acagctaatt gaaaaaatga atctgtctgc
      481 tattcaggat agagaaattt gctgctattc aatttcttgc aaagaaaagg ataatataga
      541 tatcacactt cagtggctta ttcagcattc aaaatctaga agaagcgacc cagctttctt
      601 gtacaaagtg gttgatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      661 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      721 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      781 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      841 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
      901 caagggggag gattgggaag acaatagcag gcatgctggg ggatgcggtg ggctctatgg
      961 cttctga
//
