LOCUS       sequence-119574-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArl4D with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARL4D-V5-His6
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(635..659)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             685..726
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             736..753
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(776..793)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    782..893
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaaagcag gcttagaagg agatagaacc atggggaacc acttgactga gatggcgccc
       61 actgcctcct ccttcttgcc ccacttccaa gccctgcatg tcgtggtcat tgggctggac
      121 tctgctggaa agacctccct cctttaccgc ctcaagttca aggagtttgt ccagagtgtc
      181 cccaccaaag gcttcaacac cgagaagatc cgggtgcccc tcgggggatc gcgtggcatc
      241 accttccaag tgtgggacgt cggggggcag gagaagctgc gaccactgtg gcgctcttat
      301 acccgccgga cagacggtct agtgtttgtg gtggacgctg cggaggctga gcggctggag
      361 gaagccaagg tggagttgca ccgaatcagc cgggcctcgg acaaccaggg cgtgccagtg
      421 ctggtgctgg ccaacaagca ggaccagccc ggggcactga gcgctgctga ggtggagaag
      481 aggctggcag tccgagagct agcagccgcc actctcactc atgtgcaagg ctgcagcgct
      541 gtggacggtc tgggcctgca gcagggcctt gagcgcctct atgagatgat cctcaagagg
      601 aagaaggcag ctcggggtgg caagaagaga cgggacccag ctttcttgta caaagtggtt
      661 gatctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
      721 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
      781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
      961 tgggaagac
//
