LOCUS       sequence-119576-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArfrp with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-ARFRP1-V5-His6
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(634..658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             684..725
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             735..752
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(775..792)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    781..1005
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgtacacgct gctgtcgggc ttgtacaagt
       61 acatgtttca gaaggacgag tactgcatcc tgatcctggg cctggacaat gctgggaaga
      121 cgaccttcct ggagcagtcg aaaacccgat ttaacaagaa ctacaagggg atgagtctat
      181 ccaaaatcac caccaccgtg ggcctaaaca tcggcactgt ggatgtggga aaggctcggc
      241 tcatgttctg ggacttagga gggcaggaag agctgcagtc tttgtgggac aagtattatg
      301 cggagtgtca cggcgtcatc tacgtcattg actccaccga cgaggagagg ctggctgagt
      361 ccaagcaggc gtttgagaag gtggtgacca gcgaggcgct gtgcggtgtc cccgtcttgg
      421 tgctggccaa caagcaggat gtggagacgt gcctctcaat ccctgacatc aagacggcct
      481 tcagcgactg caccagcaag atcggcaggc gcgattgcct gacccaggcc tgctcggccc
      541 tcacaggcaa aggggtgcgc gagggcatcg agtggatggt gaagtgtgtc gtgcggaatg
      601 tgcaccggcc gccgcggcag agggacatca cggacccagc tttcttgtac aaagtggttg
      661 atctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
      721 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
      781 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      841 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      901 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      961 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
//
