LOCUS       sequence-119578-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hSar1 with C-term V5 and His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST40-SAR1-V5-His6
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(625..649)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             675..716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             726..743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(766..783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    772..883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aaaaagcagg cttagaagga gatagaacca tgtctttcat ctttgagtgg atctacaatg
       61 gcttcagcag tgtgctccag ttcctaggac tgtacaagaa atctggaaaa cttgtattct
      121 taggtttgga taatgcaggc aaaaccactc ttcttcacat gctcaaagat gacagattgg
      181 gccaacatgt tccaacacta catccgacat cagaagagct aacaattgct ggaatgacct
      241 ttacaacttt tgatcttggt gggcacgagc aagcacgtcg cgtttggaaa aattatctcc
      301 cagcaattaa tgggattgtc tttctggtgg actgtgcaga tcattctcgc ctcgtggaat
      361 ccaaagttga gcttaatgct ttaatgactg atgaaacaat atccaatgtg ccaatcctta
      421 tcttgggtaa caaaattgac agaacagatg caatcagtga agaaaaactc cgtgagatat
      481 ttgggcttta tggacagacc acaggaaagg ggaatgtgac cctgaaggag ctgaatgctc
      541 gccccatgga agtgttcatg tgcagtgtgc tcaagaggca aggttacggc gagggtttcc
      601 gctggctctc ccagtatatt gacgacccag ctttcttgta caaagtggtt gatctagagg
      661 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
      721 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
      781 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      841 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      901 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
//
