LOCUS       sequence-119581-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 IL1B
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagttgggt aggaagacac aaattgcatg gtgaagtcag ttatatcctg gccgcctttg
      241 gtccctccca ggaagacggg catgttttct gcttgagagg tgctgatgta ccagttgggg
      301 aactgggcag actcaaattc cagcttgtta ttgatttcta tcttgttgaa gacaaatcgc
      361 ttttccatct tcttctttgg gtaatttttg ggatctacac tctccagctg tagagtgggc
      421 ttatcatctt tcaacacgca ggacaggtac agattctttt ccttgaggcc caaggccaca
      481 ggtattttgt cattactttc ttctccttgt acaaaggaca tggagaacac cacttgttgc
      541 tccatatcct gtccctggag gtggagagct ttcagttcat atggaccaga catcaccaag
      601 cttttttgct gtgagtcccg gagcgtgcag ttcagtgatc gtacaggtgc atcgtgcaca
      661 taagcctcgt tatcccatgt gtcgaagaag ataggttctt cttcaaagat gaagggaaag
      721 aaggtgctca ggtcattctc ctggaaggtc tgtgggcagg gaaccagcat cttcctcagc
      781 ttgtccatgg ccacaacaac tgacgcggcc tgcctgaagc ccttgctgta gtggtggtcg
      841 gagattcgta gctggatgcc gccatccaga gggcagaggt ccaggtcctg gaaggagcac
      901 ttcatctgtt tagggccatc agcttcaaag aacaagtcat cctcattgcc actgtaataa
      961 gccatcattt cactggcgag ctcaggtact tctgccatgc caactttttt tgtaaaaact
//
