LOCUS       sequence-119582-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf1 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF1
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(581..605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(649..677)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaaaagcag gcttagaagg agatagaacc atggggaaca tcttcgccaa cctcttcaag
       61 ggcctttttg gcaaaaaaga aatgcgcatc ctcatggtgg gcctggatgc tgcagggaag
      121 accacgatcc tctacaagct taagctgggt gagatcgtga ccaccattcc caccataggc
      181 ttcaacgtgg aaaccgtgga gtacaagaac atcagcttca ctgtgtggga cgtgggtggc
      241 caggacaaga tccggcccct gtggcgccac tacttccaga acacacaagg cctgatcttc
      301 gtggtggaca gcaatgacag agagcgtgtg aacgaggccc gtgaggagct catgaggatg
      361 ctggccgagg acgagctccg ggatgctgtc ctcctggtgt tcgccaacaa gcaggacctc
      421 cccaacgcca tgaatgcggc cgagatcaca gacaagctgg ggctgcactc actacgccac
      481 aggaactggt acattcaggc cacctgcgcc accagcggcg acgggctcta tgaaggactg
      541 gactggctgt ccaatcagct ccggaaccag aagtgatagg acccagcttt cttgtacaaa
      601 gtggttcgat ctagaatggc tagcaaagga gaagaacttt tcactggagt tgtcccaatt
      661 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      721 ggtgatgcta catacggaaa gcttaccctt aaatttattt gcactactgg aaaactacct
      781 gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt ttcccgttat
      841 ccggatcata tgaaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      901 ggaacgcact atatctttca aagatgacgg g
//
