LOCUS       sequence-119583-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf3 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF3
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(581..605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(649..677)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaaaagcag gcttagaagg agatagaacc atgggcaata tctttggaaa ccttctcaag
       61 agcctgattg ggaagaagga gatgcgcatc ctgatggtgg gcctggatgc cgcaggaaag
      121 accaccatcc tatacaagct gaaactgggg gagatcgtca ccaccatccc taccattggg
      181 ttcaatgtgg agacagtgga gtataagaac atcagcttta cagtgtggga tgtgggtggc
      241 caggacaaga ttcgacccct ctggagacac tacttccaga acacccaagg gttgatattt
      301 gtggtcgaca gcaatgatcg ggagcgagta aatgaggccc gggaagagct gatgagaatg
      361 ctggcggagg acgagctccg ggatgctgta ctccttgtct ttgcaaacaa acaggatctg
      421 cctaatgcta tgaacgctgc tgagatcaca gacaagctgg gcctgcattc ccttcgtcac
      481 cgtaactggt acattcaggc cacctgtgcc accagcgggg acgggctgta cgaaggcctg
      541 gactggctgg ccaatcagct caaaaacaag aagtgatagg acccagcttt cttgtacaaa
      601 gtggttcgat ctagaatggc tagcaaagga gaagaacttt tcactggagt tgtcccaatt
      661 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      721 ggtgatgcta catacggaaa gcttaccctt aaatttattt gcactactgg aaaactacct
      781 gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt ttcccgttat
      841 ccggatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      901 gaacgcacta tatctttcaa agatgacggg
//
