LOCUS       sequence-119584-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf4 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF4
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(578..602)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(646..674)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaaaagcag gcttagaagg agatagaacc atgggcctca ctatctcctc cctcttctcc
       61 cgactatttg gcaagaagca gatgcgcatt ttgatggttg gattggatgc tgctggcaag
      121 acaaccattc tgtataaact gaagttaggg gagatagtca ccaccattcc taccattggt
      181 tttaatgtgg aaacagtaga atataagaac atttgtttca cagtatggga tgttggtggt
      241 caagatagaa ttaggcctct ctggaagcat tacttccaga atacccaggg tcttattttt
      301 gtggtagata gcaacgatcg tgaaagaatt caggaagtag cagatgagct gcagaaaatg
      361 cttctggtag atgaattgag agatgcagtg ctgctacttt ttgcaaacaa acaggatttg
      421 ccaaatgcta tggccatcag tgaaatgaca gataaactag ggcttcagtc tcttcgtaac
      481 agaacatggt atgttcaagc cacttgtgca acacaaggaa ctggtctgta tgaaggactt
      541 gactggctgt caaatgagct ttcaaaacgt taataggacc cagctttctt gtacaaagtg
      601 gttcgatcta gaatggctag caaaggagaa gaacttttca ctggagttgt cccaattctt
      661 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      721 gatgctacat acggaaagct tacccttaaa tttatttgca ctactggaaa actacctgtt
      781 ccatggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc ccgttatccg
      841 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      901 cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa
      961 ggtgataccc cttgttaatc gtatcgagtt aaaaggtatt gatttttaaa gaagatggaa
     1021 acattctcgg acccaacctc gagtac
//
