LOCUS       sequence-119585-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of hArf5 with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pDEST47-ARF5
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(578..602)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(646..674)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaaaagcag gcttagaagg agatagaacc atgggcctca ccgtgtccgc gctcttttcg
       61 cggatcttcg ggaagaagca gatgcggatt ctcatggttg gcttggatgc ggctggcaag
      121 accacaatcc tgtacaaact gaagttgggg gagattgtca ccaccatccc aaccataggc
      181 ttcaatgtag aaacagtgga atataagaac atctgtttca cagtctggga cgtgggaggc
      241 caggacaaga ttcggcctct gtggcggcac tacttccaga acactcaggg cctcatcttt
      301 gtggtggaca gtaatgaccg ggagcgggtc caagaatctg ctgatgaact ccagaagatg
      361 ctgcaggagg acgagctgcg ggatgcagtg ctgctggtat ttgccaacaa gcaggacatg
      421 cccaacgcca tgcccgtgag cgagctgact gacaagctgg ggctacagca cttacgcagc
      481 cgcacgtggt atgtccaggc cacctgcgcc acccaaggca caggtctgta cgatggtctg
      541 gactggctgt cccacgagct gtcaaagcgc taataggacc cagctttctt gtacaaagtg
      601 gttcgatcta gaatggctag caaaggagaa gaacttttca ctggagttgt cccaattctt
      661 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
      721 gatgctacat acggaaagct tacccttaaa tttatttgca ctactggaaa actacctgtt
      781 ccatggccaa cacttgtcac tactttctct tatggtgttc aatgcttttc ccgttatccg
      841 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      901 cgcactatat ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa
      961 ggtgataccc ttgttaatcg tatcgagtta aaaggtattg atttt
//
