LOCUS       sequence-119586-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ANXA1
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..54)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(197..216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(198..216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        248..352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             537..915
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(571..590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccccggggac gctctcgccc tgcttgtgcc gtggtgatgg tggtgatggt ggtgaccaga
       61 ggtgcccctg gtttcactta cgtgtttaat gtctttacag actttataca ttactgtcat
      121 ggttaaataa aataaaataa aataaaataa aataaaataa aataaaataa aataaaataa
      181 aataaactta agatgtccct atagtgagtc gtattagacg tcaggtggca cttttcgggg
      241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      301 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      361 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      421 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      481 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      541 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      601 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      661 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      721 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      781 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      841 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      901 aatggcaaca acgttgcgca aactattaac tgggcgaact acttactcta gcttcccggc
      961 aacaattaat agactggatg gaggcggata agttgcagga cccttctgcg ctcggccctt
     1021 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1081 attgcagccc tggggccaga
//
