LOCUS       sequence-119587-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV0-OCT-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..48)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(209..228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(210..228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(273..476)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(302..322)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        477..856
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctcgcccttg ctcacgaatt cagcgtaatc tggaacatcg tatgggtagg atccctggac
       61 tggcttccca taccgaaaat ttcgaaccat ggaagtgtga gcctttctaa gagctgcctt
      121 gttgagcagg attctcaaat tagacagcat ggtacctccg gatcgataat tctagagggc
      181 ccgtttaaac gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc
      241 cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt
      301 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
      361 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
      421 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
      481 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
      541 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
      601 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
      661 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
      721 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
      781 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
      841 gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc
      901 ctaaccctaa gcaga
//
