LOCUS       sequence-119588-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 ANXA1
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtaccacg cgtacccaac acacggacac atgcacgaac acgcatgcag agagcatcat
       61 acatcatcta tagagaagta cacgtaaaag taagactcgt cgtctgccag ctacaaccga
      121 gaaggcgacg tgaaaggaac aagaaaggag gaggagggcg gccgcttaca ccactttgta
      181 caagaaagtt gggtagtttc ctccacaaag agccaccagg attttctcat aatctccttt
      241 ggtttcatcc aggatggctt ggcaaaggga gataccatac atcttctgat agaatgcttt
      301 gatatcattc atgtcaattt cagaacggga aaccataatc ctgatcaatg ccttatggcg
      361 agttccaaca cctttcatgg cttgatgaag cttctctgca aagaaagctg gtttgcttgt
      421 ggcgcacttc acgatagctg tgaggcattt ctcaatgtca cctttcaact ccaggtccag
      481 aactttgttc atgtcatgct tactgtactt ggtgtatttc tgaaacactc tgcgaagttg
      541 tggatagctt ctggtggtaa ggatggtatt gaacacgttt acgtctgtcc cctttctcct
      601 ttctcctgct tcatacaagg ccctggcatc tgaatcagcc aagtcttcat tcacaccaaa
      661 gtcctcagat cggtcaccct tagcaagaga aagcaaagcg ttccgaaaat ctccagatgt
      721 gtctgaggtt atgtctttgg ccagatctct cttcagttcc tctctgtaga ccctgttaat
      781 gtctctgatt tctttgttag ttcttgatgc caaaatctca attagagtat cttcatcagt
      841 tccaaggccc ttcatggcag cacgaagttc atcagcatca aattgcgctg gagtttttag
      901 cagagctaaa acaacctcct caaggtgacc tgtaagggct ttcttcagtg tttcatccag
      961 gggctttcct gtttcctgga gatatgctgc tttgatctgt tgacgctgtg cattgtttcg
     1021 cttagttaga atgtcaatga tggttgcttc atccacacct ttaaccatta tggccttatg
     1081 ca
//
