LOCUS       sequence-119590-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 EBNA1BP2
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtaccacg cgtacccaac acacggacac atgcacgaac acgcatgcag agagatcata
       61 catcatctat agagaagtac acgtaaaagt aagactcgtc gtctgccagc tacaaccgag
      121 aaggcgacgt gaaaggaaca agaaaggagg aggagggcgg ccgcttacac cactttgtac
      181 aagaaagttg ggtagtgtgt tctgttcttc atcttctctc ttgttcgttt tccaggtctc
      241 ttatttgacc ctttcttgcc aggcctcttg aggcctctgc catgagctgt cttggcccgg
      301 aagctagata catcatcata gctctcccga gtgttccact ttgagccttt cttctttcca
      361 ccaaaaccaa acttctggtt tttataccgt cgtttagcac tgggcccctt cctcatctgc
      421 tggcctttgg ctcctgcctt cttgcgctgt gccagaggtt tctgatctcc ctcaaggaaa
      481 tccagtttat cagagaagcc tttctgatat ttcttaatag cattcatcat atgggctttc
      541 tcctgctgcc tcttctgaag aacctccgtt tgcaccttct tcccgtattt cctaagtgct
      601 cgcagttgct tagctttttc agacctctcc atggcagcct gtttagtctg cagcttctgt
      661 cgaatcttct gcatctgcag atcagatttg gccatttccg caaaataatc agtgggtcgc
      721 ttcgtaggga ctttgagctg atggaggcgg ggtaagactg caagcactgc ggcctgggct
      781 tggcgataga aactcatctc tcgctggaag tcgtcttctg gatcaacagc tttctggtcc
      841 ttgttctgag gtgctggcgc ctcagatcca ccgatctccg gtaccggacc cagtgtcaca
      901 tcgagccttt caacccattc cagatcccgc ttgaattctg ccaaacattg cttcaggcca
      961 ttcacgtcgt tcacggcctt cttcggcccc tctagcacga cattgaggcc tggcttcaga
     1021 agccctcggg aaaacgcatc ctgcaactct ctgtctg
//
