LOCUS       sequence-119591-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV1-OCT-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..43)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(204..223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(205..223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(268..471)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(297..317)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     706..725
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        800..904
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccttgctcac gaattcagcg taatctggaa catcgtatgg gtaggatccc tggactggct
       61 tcccataccg aaaatttcga accatggaag tgtgagcctt tctaagagct gccttgttga
      121 gcaggattct caaattagac agcatggtac ctccggatcg ataattctag agggcccgtt
      181 taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
      241 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      301 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
      361 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
      421 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
      481 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgacgcg
      541 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca
      601 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag
      661 cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga
      721 ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga cgtcaggtgg
      781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      961 tcctggtttt gctcacccag aaacgctggt gaaagtaaa
//
