LOCUS       sequence-119596-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets HA-eGFP to mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Kerr SC, Kahn RA
  TITLE     Tool box: Plasmids for the expression or knockdown of human ARF 
            Family GTPases (ARF/ARL/SAR) and their co-expression in bacteria 
            with N-myristoyltransferases.
  JOURNAL   Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.
  PUBMED    27057421
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2015-09-21"; volume: "5(3):e1090523. doi"; pages: "
            10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep"
COMMENT     Sequence Label: pCMV5-OCT-HA-eGFP
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..43)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(204..223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(205..223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(268..319)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(297..317)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     513..532
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        607..711
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(931..950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccttgctcac gaattcagcg taatctggaa catcgtatgg gtaggatccc tggactggct
       61 tcccataccg aaaatttcga accatggaag tgtgagcctt tctaagagct gccttgttga
      121 gcaggattct caaattagac agcatggtac ctccggatcg ataattctag agggcccgtt
      181 taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
      241 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
      301 cctaccgccc atttgcgtca atggggcgga gttgttacga caacgcgtat atctggcccg
      361 tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt
      421 caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac
      481 acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt
      541 gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga
      601 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      661 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      721 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      781 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      841 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      901 tttccaatga tgagcacttt ttaaagttct gctatgtggc gcggtattat cccgtattga
      961 cgccgggcaa gagcaactcg gtcgccgcat acactattct ca
//
