LOCUS       sequence-119598-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TFAP4
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgctcgt ctgccagcta caaccgagaa
      121 ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca ctttgtacaa
      181 gaaagttggg caggcaagct ccccgtcccc cgacggctcc tcccggctct ggtccatggc
      241 gtcactgtct gaggcctcgg agtcggaggc ggtgtcagag gtgggggcct ccgggcagct
      301 gcggacaggc ttcacgatga cagctcgccg ctgctcctcc tccagctcct gcttttcctg
      361 ggtgccctcg atgtgctgga ttgcctgcac gatggtgtcc agattttgcc gggatgtgga
      421 aacagagttg atgaccgagg aggggcccat ggtgacgaca ttgatgtggt gggagggagg
      481 aggaggcggt gctggcacga tcaccgtggg gtggtgggtg ggggccggag ggggcagaag
      541 ctgggtccgc agctgctgct gttcccgctc cagcttctcc tggtgcagca gcctcacctg
      601 ttcctgctgc tgctgcagct gcacctgctg cgcaatcacc ttgagctttt ccgggtacat
      661 gtgggcctcc agcgagcgca cctgctcctc cagcatcatg cgcaccgagc gctccttgtc
      721 cagctgctgc cgcagctcaa tcatctcccg ccgcaggtcc tccgccttct cgtcctccca
      781 gatgtccggg gagcctatgc cttcgtcctt gtcctctgcc cgccgtcgct tgggggacga
      841 gccgctcagc tcctggatga agcgcttgag ctgtgtgttc tgctgcaaga gcctggtctt
      901 ctcctgctcc a
//
