LOCUS       sequence-119599-       12383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible human expression vector that adds a ""flexible"" 
            3XFLAG,V5,YFP N terminus tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Mita P, Lhakhang T, Li D, Eichinger DJ, Fenyo D, Boeke JD
  TITLE     Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for 
            High-Throughput IP.
  JOURNAL   Biol Proced Online. 2016 Aug 15;18:16. doi: 
            10.1186/s12575-016-0046-x. eCollection 2016.
  PUBMED    27528826
REFERENCE   2  (bases 1 to 12383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Proced
            Online."; date: "2016-08-15"; volume: "18:16. doi"; pages: " 
            10.1186/s12575-016-0046-x. eCollection 2016"
COMMENT     Sequence Label: HuEV-A
FEATURES             Location/Qualifiers
     source          1..12383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     289..313
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    376..409
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             416..487
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             488..529
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    530..563
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(566..568,569..571,572..1282)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(611..632)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(872..891)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1219..1240
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1289..1309
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1310..1333
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    1334..1367
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1371..1404
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    1412..1536
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1561..1566,1567..1584,1585..1591)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1645..2304
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1711..1730)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2646..2951
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2830..2849
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2992..3116)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    3178..3312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3202..3221)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3256..3275
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3455..3477)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4039..5828
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8492..8510)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8578..8682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8683..8751,8752..9543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8901..8920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9714..10302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10203..10222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10497..10996
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10532..10555)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10929..10948
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11036..11635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11036..11055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11532..11552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
       61 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      181 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      301 gaaccgtcag atcgcaaatt gagcccgcag cctcccgctt cgctctctgc tcctcctgtt
      361 cgacagtcag ccgcagaagt tcttattctc tagaaagtat aggaacttca caatggacta
      421 caaggacgac gacgacaagg attataaaga tgatgatgat aaagactata aggatgacga
      481 tgacaaaggc aagcccatcc ccaaccccct gctgggcctg gacagcacct accgttcgta
      541 taatgtatgc tatacgaagt tatccatggt gagcaagggc gaggagctgt tcaccggggt
      601 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      661 tgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
      721 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
      781 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      841 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      901 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      961 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1021 tatcaccgcc gacaagcaga agaacggcat caaggccaac tttaagatcc gccacaacat
     1081 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1141 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     1201 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1261 cggcatggac gagctgtaca agcacgtgga aaacctgtat tttcagggcc tggaagttct
     1321 gttccagggg cccataactt cgtataatgt atgctatacg aagttatcga gaagttccta
     1381 ttctctagaa agtataggaa cttcgagatc aacaagtttg tacaaaaaag ctgaacgaga
     1441 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1501 ataatactgt aaaacacaac atatccagtc actatgtcgg ccgcattagg caccccaggc
     1561 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     1621 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1681 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1741 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1801 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1861 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1921 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1981 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     2041 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     2101 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     2161 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2221 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2281 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag
     2341 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     2401 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     2461 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     2521 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     2581 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     2641 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     2701 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     2761 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     2821 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     2881 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     2941 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     3001 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     3061 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatg
     3121 gatccggtac cagctgctag caagcttgct agcggccgct cgaggccggc aaggccggat
     3181 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3241 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3301 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3361 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg
     3421 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3481 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag gcgtcagcgg
     3541 gtgttggcgg gtgtcggggc gcagccatga ggtcgatcga ctctagagga tcgatgcccc
     3601 gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat
     3661 gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg
     3721 gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt
     3781 gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg
     3841 caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg
     3901 acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg
     3961 ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag
     4021 gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc
     4081 agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag
     4141 ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt
     4201 tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg
     4261 gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc
     4321 cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct
     4381 cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg
     4441 gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt
     4501 aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt
     4561 aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc
     4621 aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg
     4681 ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca
     4741 ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa
     4801 caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc
     4861 acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa
     4921 ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga
     4981 atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga
     5041 caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg
     5101 aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc
     5161 tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5221 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5281 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5341 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt
     5401 agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga
     5461 tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     5521 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     5581 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     5641 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     5701 tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga
     5761 gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc
     5821 ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg
     5881 catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga
     5941 gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg
     6001 aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg
     6061 gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa
     6121 caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc
     6181 atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa
     6241 atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt
     6301 tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa
     6361 aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc
     6421 aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag
     6481 ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc
     6541 ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct
     6601 taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgcttttc
     6661 caaaccaccc tccttttttg cgccctgcct ccatcaccct gaccccgggg tccagtgctt
     6721 gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc
     6781 tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg
     6841 aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga
     6901 ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct ggctctttca
     6961 cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact acctcctcga
     7021 ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc ccggcctcca
     7081 cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     7141 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     7201 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     7261 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
     7321 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgcc
     7381 cctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc
     7441 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     7501 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     7561 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     7621 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     7681 gctcctgccc ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     7741 cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc tgttccaccg
     7801 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
     7861 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
     7921 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
     7981 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
     8041 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
     8101 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
     8161 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
     8221 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
     8281 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
     8341 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
     8401 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
     8461 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8521 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8581 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8701 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8761 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8821 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8881 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     8941 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9001 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9061 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9121 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9181 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     9241 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9301 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9361 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9421 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9481 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9541 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9601 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9661 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9721 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9781 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9841 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     9901 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9961 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10021 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10081 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10141 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10201 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10261 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10321 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
    10381 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
    10441 ataatgggga aggccatcca gcctcgcgtc gaattcgtcg acctcgaaat tctaccgggt
    10501 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
    10561 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
    10621 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
    10681 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
    10741 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
    10801 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
    10861 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
    10921 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
    10981 atctccgggc ctttcgacct gcatccatct agatctcgag cagctgaagc ttaccatgac
    11041 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
    11101 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
    11161 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    11221 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
    11281 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    11341 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    11401 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    11461 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    11521 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    11581 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
    11641 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatgatatc
    11701 agatccccgg gatgcagaaa ttgatgatct attaaacaat aaagatgtcc actaaaatgg
    11761 aagtttttcc tgtcatactt tgttaagaag ggtgagaaca gagtacctac attttgaatg
    11821 gaaggattgg agctacgggg gtgggggtgg ggtgggatta gataaatgcc tgctctttac
    11881 tgaaggctct ttactattgc tttatgataa tgtttcatag ttggatatca taatttaaac
    11941 aagcaaaacc aaattaaggg ccagctcatt cctcccactc atgatctata gatctataga
    12001 tctctcgtgg gatcattgtt tttctcttga ttcccacttt gtggttctaa gtactgtggt
    12061 ttccaaatgt gtcagtttca tagcctgaag aacgagatca gcagcctctg ttccacatac
    12121 acttcattct cagtattgtt ttgccaagtt ctaattccat cagaagctgg tcgaccaatt
    12181 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
    12241 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
    12301 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
    12361 tatcataata tgtacgcgtc cgc
//
