LOCUS       sequence-119616-        3952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, ColE1 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 59
FEATURES             Location/Qualifiers
     source          1..3952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      136..222
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        317..397
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             836..858
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     rep_origin      1485..1943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1572..1591)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1785..1806
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(2053..2641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2133..2152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2812..3603,3604..3672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3435..3454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3673..3777)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3855..3873
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcggccgcgc gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
       61 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      121 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      181 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      241 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      301 gaatttgata gagataagtc cagccttgca agaagcggat acaggagtgc aaaaaatggc
      361 tatctctaga aaggcctacc ccttaggctt tatgcaacag aaacaataat aatggagtca
      421 tgaccatgac aatgcacctg gggctcgact atatagatag tctcgttgaa gaagatgaga
      481 acgagggcat ctaccgctgc aagcgcgaga tgttcaccga ccctcggctg ttcgatttag
      541 agatgaaaca catctttgag ggcaactgga tttatctcgc ccacgagagc cagattcccg
      601 agaagaacga ctattacacc acgcagatgg gccggcagcc gatattcatc acacgcaaca
      661 aagatggtga gctgaatgcc ttcgtcaatg cctgaattcg agtgaataaa aggttttggg
      721 cagcgcgcca atggcggcgc gtatgtccag ggataaaggc gtccagcggt gcgtaagcac
      781 cgccgggcgc tttttttttg cgcgaattcc caattcggtt cacccgggaa ataattttgt
      841 ttaactttaa gaaggagata tacatatgcc ctttcatatt ggaagcggat gtcttcccgc
      901 caccatcagt aatcgccgca tttatcgtat tgcctggtct gatacccccc ctgaaatgag
      961 ttcctgggaa aaaatgaagg aatttttttg ctcaacgcac cagactgaag cgctggagtg
     1021 catctggacg atttgtcacc cgccggccgg aacgacgcgg gaggatgtga tcaacagatt
     1081 tgaactgctc aggacgctcg cgtatgccgg atgggaggaa agcattcatt ccggccagca
     1141 cggggaaaat tacttctgta ttctggatga agacagtcag gagatattgt cagtcaccct
     1201 tgatgatgcc gggaactata ccgtaaattg ccaggggtac agtgaaacac atcgcctcac
     1261 cctggacaca gcacagggtg aggagggcac aggacacgcg gaaggggcat ccgggctgca
     1321 gatatccatg gtaccgagct cggatccgtc gacaagcttg cggccgccca gcttgctggc
     1381 gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct
     1441 aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg
     1501 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1561 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1621 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1681 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggcggccat cgccctgata
     1741 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1801 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     1861 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1921 caaaatatta acgtttacaa tttctaaccg atccccctgg agcttggaat caggggataa
     1981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2461 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2701 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2761 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2821 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2881 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2941 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3001 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3061 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3121 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3181 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3241 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3301 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3361 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3421 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3481 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3541 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3601 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3661 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3721 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3781 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3841 taaaaataga taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttggtcgcat
     3901 cgatgctggc ttatcgaaat taatacgact ccactatagg gagaccggaa tt
//
