LOCUS       sequence-119628-        8005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  -The prostate specific promoter controls expression of FlpO linked 
            to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 
            SMAD4 ex2. are expressed by the U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8005)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2,...
FEATURES             Location/Qualifiers
     source          1..8005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        698..946
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        967..1042
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1055..1303
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1324..1399
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1412..1660
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1681..1756
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1769..2017
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2038..2113
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      2613..2622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2619..2621,2622..2624,2625..3335)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2664..2685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2925..2944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3272..3293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3351..3407
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3414..3419,3420..3440,3441..4712)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     polyA_signal    4816..4937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4853..4872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5021..5038)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5027..5251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(5268..5301)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(5784..5800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5784..5800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5797..5819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5808..5824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5832..5838,5839..5856,5857..5862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5877..5898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6015..6032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6186..6774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6266..6285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6945..7736,7737..7805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7568..7587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7806..7910)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7978..7996
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cagatctgat atctctagag
      421 aagtcgacgt ttaaagtacc ctacagttga agtcggaagt ttacatacac ttaagttgga
      481 gtcattaaaa ctcgtttttc aactactcca caaatttctt gttaacaaac aatagttttg
      541 gcaagtcagt taggacatct actttgtgca tgacacaagt catttttcca acaattgttt
      601 acagacagat tatttcactt ataattcact gtatcacaat tccagtgggt cagaagttta
      661 catacactaa gttgactgtg cctttaaaca gtcgcgagag ggcctatttc ccatgattcc
      721 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
      781 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
      841 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
      901 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgcag caattcactg
      961 taaagcgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     1021 aaaagtggca ccgagtcggt gcttttttac cggtgagggc ctatttccca tgattccttc
     1081 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     1141 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     1201 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     1261 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgtgcgtg tttgtgcctg
     1321 tccgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     1381 agtggcaccg agtcggtgct tttttatcga tgagggccta tttcccatga ttccttcata
     1441 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     1501 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     1561 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     1621 tttcttggct ttatatatct tgtggaaagg acgaaacacc gaaggagaaa aaagatgaat
     1681 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     1741 ggcaccgagt cggtgctttt ttggtaccga gggcctattt cccatgattc cttcatattt
     1801 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     1861 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     1921 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     1981 cttggcttta tatatcttgt ggaaaggacg aaacaccgtg caacagctcc tgcatgggtt
     2041 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     2101 accgagtcgg tgcttttttg agctcactag ttatgatagc atcttgttct tagtcttttt
     2161 cttaataggg acataaagcc cacaaataaa aatatgcctg aagaatggga caggcattgg
     2221 gcattgtcca tgcctagtaa agtactccaa gaacctattt gtatactaga tgacacaatg
     2281 taatagtcat catgtttaaa catctaccat tccagttaag aaaatatgat agcatcttgt
     2341 tcttagtctt tttcttaata gggacataaa gcccacaaat aaaaatatgc ctgaagaatg
     2401 ggacaggcat tgggcattgt ccatgcctag taaagtactc caagaaccta tttgtatact
     2461 agatgacaca atgtcaatgt ctgtgtacaa ctgccaactg ggatgcaaga cactgcccat
     2521 gccaatcatc ctgaaaagca gctataaaaa gcaggaagct actctgcacc ttgtcagtga
     2581 ggtccagata cctacagatt aattaacccg gggccaccat ggtgagcaag ggcgaggagc
     2641 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2701 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     2761 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgtcctggg
     2821 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     2881 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     2941 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3001 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacttta
     3061 gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3121 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     3181 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
     3241 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3301 ccgggatcac tctcggcatg gacgagctgt acaaggtata cggaagcgga gctactaact
     3361 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctgct agcatggctc
     3421 ctaagaagaa gaggaaggtg atgagccagt tcgacatcct gtgcaagacc ccccccaagg
     3481 tgctggtgcg gcagttcgtg gagagattcg agaggcccag cggcgagaag atcgccagct
     3541 gtgccgccga gctgacctac ctgtgctgga tgatcaccca caacggcacc gccatcaaga
     3601 gggccacctt catgagctac aacaccatca tcagcaacag cctgagcttc gacatcgtga
     3661 acaagagcct gcagttcaag tacaagaccc agaaggccac catcctggag gccagcctga
     3721 agaagctgat ccccgcctgg gagttcacca tcatccctta caacggccag aagcaccaga
     3781 gcgacatcac cgacatcgtg tccagcctgc agctgcagtt cgagagcagc gaggaggccg
     3841 acaagggcaa cagccacagc aagaagatgc tgaaggccct gctgtccgag ggcgagagca
     3901 tctgggagat caccgagaag atcctgaaca gcttcgagta caccagcagg ttcaccaaga
     3961 ccaagaccct gtaccagttc ctgttcctgg ccacattcat caactgcggc aggttcagcg
     4021 acatcaagaa cgtggacccc aagagcttca agctggtgca gaacaagtac ctgggcgtga
     4081 tcattcagtg cctggtgacc gagaccaaga caagcgtgtc caggcacatc tactttttca
     4141 gcgccagagg caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc
     4201 ccgtgctgaa gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc
     4261 tgctgaagga caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc
     4321 ccatcttcgc tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct
     4381 ttctgagcat gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga
     4441 gggcctccgc cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact
     4501 acttcgccct ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc
     4561 tgaaggacga gaccaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg
     4621 ccgagggcag catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact
     4681 acctgagcag ctacatcaac aggcggatct aaatgcatca gccataccac atttgtagag
     4741 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4801 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4861 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4921 ctcatcaatg tatcttaacg cgtaaattgt aagcgggccg gccatatttg tcaccggact
     4981 gcggccgcga ctctagatca taatcagccc gggggatcag cctcgactgt gccttctagt
     5041 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5101 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5161 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5221 aggcatgctg gggatgcggt gggctctatg gaaccagctg gggcgcgata acttcgtata
     5281 ggagacttta tacgaagtta tgcgccattc tagttgtggt ttgtccaaac tcatcaatgt
     5341 atcttatcat gtctggatcc catcacaaag ctctgacctc aatcctatag aaaggaggaa
     5401 tgagccaaaa ttcacccaac ttattgtggg aagcttgtgg aaggctactc gaaatgtttg
     5461 acccaagtta aacaatttaa aggcaatgct accaaatact aattgagtgt atgttaactt
     5521 ctgacccact gggaatgtga tgaaagaaat aaaagctgaa atgaatcatt ctctctacta
     5581 ttattctgat atttcacatt cttaaaataa agtggtgatc ctaactgacc ttaagacagg
     5641 gaatctttac tcggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa tgtatttggc
     5701 taaggtgtat gtaaacttcc gacttcaact gtaggggatc ctctagagtc gacctgcagg
     5761 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5821 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5881 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5941 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6001 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6061 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6361 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6601 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7921 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7981 aggcgtatca cgaggccctt tcgtc
//
