LOCUS       sequence-119632-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 GATA1
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    164..188
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccacgcgtac ccaacacacg gacacatgca cgaacacgca tgcagagaga tcatacatca
       61 tctatagaga agtacacgta aaagtaagac tcgtcgtctg ccagctacaa ccgagaaggc
      121 gacgtgaaag gaacaagaaa ggaggaggag ggcggccgct tacaccactt tgtacaagaa
      181 agttggggaa gccagggggt gcctggggcc cagagacttg ggttgtccag aattctggct
      241 acaagaggag aaggacacca cccctcctct ggaggccatg ctctgtgccc tcatgagctg
      301 agcggagcca ccacagtagt gctggtggtg tagcttgtag tagaggccgc aggcattgca
      361 cacgggatcc ccactggcat ttctccgcca cagtgtcgtg gtggtcgtct ggcagttggt
      421 gcactgagta cctgcccgtt tactgacaat caggcgcttc ttgggccgga tgaggggcct
      481 gttctgccca ttcatcttgt gatagaggcc gcaggcgttg cataggtagt ggcctgtcct
      541 gtccctccgc cacagtggag tggctgttgc tccgcagttc acacactccc tggcctcaca
      601 gggaggcagg gggagagttc cacgaagctt gggagaggaa taggctgctg aattgagggg
      661 gctcccggtg ggagaaaaga aggtactgga aaagtcaggg cccccataag cactattggg
      721 gacagggagt gatgaaggca gtgcaggtcc cagggtcagg aggtctgggc tcagccgctc
      781 tgtcttcaaa gtctccagga agctggtgct gccttttcca tccagatctt ccacggcctg
      841 gggaggagag tcctcgcggg tgggacacac agttgaggca gggtagagcc ccgtcttgcc
      901 gtaggcccag ccggcatatg gtgagccccc tgggatcccc tccatacagt tgagcaatgg
      961 gtacacctga aagactgggg agtgtctgta ggcctcagcg tccctgtagt aggccagtgc
     1021 cgcagctgca gcggtggctg tgctcggggc agtggaggaa gctgctgcat ccaagccctc
     1081 aggcccagag gggaagaaaa cccctgattc tggtgtggag
//
