LOCUS       sequence-119634-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 USF2
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taccacgcgt acccaacaca cggacacatg cacgaacacg catgcagaga gatcatacat
       61 catctataga gaagtacacg taaaagtaag actcgtcgtc tgccagctac aaccgagaag
      121 gcgacgtgaa aggaacaaga aaggaggagg agggcggccg cttacaccac tttgtacaag
      181 aaagttgggc actgccgggt gccctcgccc accatctcca ggttgtgctg ctgcagctgg
      241 gctcgaagca gggcgttctc attcttcagc tcctcgatct gctgcctcag gagctcgttg
      301 tccatctgca gccgctcggc ctctttgaag gtctcctgca tgcgctggtt ggtctggcgc
      361 aactcccgga tgtaatcgca ggccttggac aggatccctc ctttactcgc tcccgtcttg
      421 ctgttgtctg cgttacagtc tggaatgatt ttcgaaagct ggacgatcca gttgttgatc
      481 ttgtccctcc gcctccgctc cacttcgttg tgctgggctc ttctcctctc atctcggggt
      541 gttctggttc catcaatttt tggagagtaa gggtgtgtcc ggggggcgat cgtcctctgt
      601 gttcctgtct gaagcacatc ctggggcgtc atcatgacgt agaactggcc tccagcctgc
      661 aagctctggt ctgtggtctg tacggacaca gccgtagtat ctcccacact ggacgctggg
      721 aaataggcaa atcgtgcctc cccgctgaca gcctcggccg ccggactgcc accattgctg
      781 aagggatttt ggatcacagc cagcgggaag ggcgctgcag gacctggggg cacagaggca
      841 gcggcggggc ccgggcgctg ggctgccccg tccacaccca cctgggtcac agcctgctgc
      901 ccccccgcga aggcagcggt ggacacgacg ctgacggcgc cagctgtgtc gccctggccg
      961 tccagctgac catcagtcac ctggactacg cggtatgtca cctgtcctcc atttgtctct
     1021 gtgcggaact ggtactggat gttgtggtcg ccgaacgccg cctgctggac gctggtgatg
     1081 gccaccgctg tctgctc
//
