LOCUS       sequence-119636-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 CREBZF
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    173..197
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcagggtac cacgcgtacc caacacacgg acacatgcac gaacacgcat gcagagagat
       61 catacatcat ctatagagaa gtacacgtaa aagtaagact cgtcgtctgc cagctacaac
      121 cgagaaggcg acgtgaaagg aacaagaaag gaggaggagg gcggccgctt acaccacttt
      181 gtacaagaaa gttggcaaca ttttaagaga agacgacgcc ttccgggcgc acgccgagca
      241 gaactccacc gacaccttat ccttgtccac atggagacag actcctcccg ccgagtcgtc
      301 ctcttccagc aggtcctgct tctgctttcc caccggcaga gcgtagtcgt ggtcaccggc
      361 gggcgagtct ctgaagagcg aggtggtcag ccgcagtccc acgccgctca gccggctcag
      421 caagcgagcc agtccagtct cgttggctaa gactgcccgt aggtagcgac tctcctcctg
      481 cagtgcctgt acgcgtttgc ccagctcccg attctcggcc cgcagctcct ggttctcggc
      541 tgccagaccc cggactcgac tctccagccc catcacgtac tccttcttct tcagtcgatt
      601 aaggcgggca gcggccgccg ccgccttccg gggactcttt gtcgccgcct ggttgttgtc
      661 gttaccgctg ccgccaccgc cgcctcctcc tggggacttt ctccgcctct tttcggcgct
      721 gccactgtca ctgctgctgc tgcagcctcc gataccgttt aacagccttt gcagcaggtc
      781 agagaagcgc tgcatttcag cagccgcggc ctcatcgtca tcgtcccctc tccacaggcc
      841 gccgctatcc gagcctccgc cagacgagga gagaggcccc ggcgagctaa gcccggggtc
      901 caggtgccag tccggttgcc tggggtccag gagatccgcc agttccagcc cagacagaaa
      961 gtccatatcc tccgtctctt cccccgggag gctggcgatc gcctcctc
//
