LOCUS       sequence-119638-        8931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a binary Agrobacterium vector carrying the HSVtk gene as a negative 
            selection marker against ectopic transformants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Khang CH, Park SY, Lee YH, Kang S
  TITLE     A dual selection based, targeted gene replacement tool for 
            Magnaporthe grisea and Fusarium oxysporum.
  JOURNAL   Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25.
  PUBMED    15893252
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2005-06"; volume: "42(6)"; pages: "483-92. Epub
            2005 Apr 25"
COMMENT     Sequence Label: pGKO1
FEATURES             Location/Qualifiers
     source          1..8931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             717..1847
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    2252..2273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2288..2293,2294..2311,2312..2318)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2326..2342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2331..2353
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2350..2366
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2350..2366
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2367..2594
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    2375..2431
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2435..2452)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2435..2451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2444..2466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2654..2678
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3978..4607
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5041..6108
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6174..6368
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6604..6626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6712..6852
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6767..6786
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6867..6884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7038..7626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7118..7137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7713..8507)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gaaattcgaa ttgggtactc
      301 aaattggttc ccgcttgacg acattccgaa acccccaatt tcgcggcttc gaatcgtggc
      361 tacccttggc cgaggttgcg atttctctgc cgtatctgac aatgatccaa ccggccggat
      421 gtgtgggagg gcgcaaaggg tgtaattggg tctaggcgtg agctgcatgt ccgatggcag
      481 aaaaagaact actaaaactc tgtgacgtcc tcagggctgg tagctattta gcttgcccct
      541 ccctcacccc acctagctca aacccaacaa aacacagcac cgaccacaaa aatctgcctc
      601 ttaccacctg ctcatcacct tttctcacat ataaagcagt gagctcagcc cgatttccat
      661 tcctcaattc aagtctatta actctctcaa agaggaaccc aatcttcaaa ggatccatgg
      721 cttcgtaccc ctgccatcaa cacgcgtctg cgttcgacca ggctgcgcgt tctcgcggcc
      781 atagcaaccg acgtacggcg ttgcgccctc gccggcagca agaagccacg gaagtccgcc
      841 tggagcagaa aatgcccacg ctactgcggg tttatataga cggtcctcac gggatgggga
      901 aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac
      961 ccgagccgat gacttactgg caggtgctgg gggcttccga gacaatcgcg aacatctaca
     1021 ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga
     1081 caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc
     1141 atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg
     1201 accgccatcc catcgccgcc ctcctgtgct acccggccgc gcgatacctt atgggcagca
     1261 tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacaa
     1321 acatcgtgtt gggggccctt ccggaggaca gacacatcga ccgcctggcc aaacgccagc
     1381 gccccggcga gcggcttgac ctggctatgc tggccgcgat tcgccgcgtt tacgggctgc
     1441 ttgccaatac ggtgcggtat ctgcagggcg gcgggtcgtg gcgggaggat tggggacagc
     1501 tttcggggac ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac
     1561 cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg
     1621 gcgacctgta caacgtgttt gcctgggcct tggacgtctt ggccaaacgc ctccgtccca
     1681 tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc
     1741 aacttacctc cgggatggtc cagacccacg tcaccacccc cggctccata ccgacgatct
     1801 gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgagtc gacctgcagg
     1861 catgcatcat tccactcaac attcaggctc ctctgcgcac gtaaagtgcc aaaggcaata
     1921 ccctgctcgg tggaatgccg ccgggcttgt cgattttacg cacatatgcg cattcttgac
     1981 ttgaagcgga ggagttcttc gttgcgggtt acagtgtttt aataaaagaa tggtcaaatc
     2041 aaactgctag atatacctgt cagacactct agttgttgac ccctatactc ttaatacatc
     2101 agacagtaca tgcatgttgc atgatgataa gctttggcaa gctgctctag ccaatacgca
     2161 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2221 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2281 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2341 aatttcacac aggaaacagc tatgacatga ttacgaattc gagctcggta cccggggatc
     2401 ctctagagtc gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
     2461 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2521 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2581 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     2641 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     2701 agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     2761 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     2821 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     2881 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     2941 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     3001 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     3061 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     3121 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     3181 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     3241 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     3301 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     3361 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     3421 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     3481 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     3541 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     3601 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     3661 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     3721 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     3781 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     3841 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     3901 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     3961 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     4021 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     4081 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     4141 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     4201 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     4261 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     4321 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     4381 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     4441 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     4501 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     4561 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     4621 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     4681 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     4741 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     4801 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     4861 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     4921 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     4981 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac
     5041 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     5101 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     5161 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     5221 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     5281 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5341 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5401 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5461 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5521 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     5581 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     5641 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     5701 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     5761 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     5821 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     5881 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5941 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     6001 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     6061 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6121 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     6181 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     6241 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     6301 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     6361 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     6421 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     6481 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     6541 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     6601 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     6661 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     6721 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     6781 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6841 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7081 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7441 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7501 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7561 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7621 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7681 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     7741 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     7801 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     7861 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     7921 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     7981 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     8041 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     8101 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     8161 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     8221 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     8281 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     8341 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     8401 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     8461 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     8521 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     8581 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     8641 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     8701 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     8761 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     8821 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     8881 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
